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Soluble fibrin species in arterial thrombi

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Author: Sandbæk, G. · Bjørnsen, S. · Sobel, J.H. · Nieuwenhuizen, W. · Matsueda, G. · Brosstad, F.
Institution: Gaubius Instituut TNO
Source:Blood Coagulation and Fibrinolysis, 1, 11, 1-5
Identifier: 235450
Keywords: Aged · Antibodies, Monoclonal · Arteries · Blotting, Western · Female · Fibrin · Fibrinogen · Fibrinopeptide A · Humans · Male · Middle Aged · Molecular Weight · Oxidation-Reduction · Protein Structure, Quaternary · Solubility · Thrombosis


The aim of this study was to characterize soluble fibrin(ogen) species in human, arterial, in-vivo-formed thrombi, using the immunoblotting technique. Specimens were collected via intra-arterial catheters in six patients scheduled for catheter-directed thrombolysis. Unreduced and reduced samples of the supernatants from the arterial thrombi-derived specimens were electrophoresed on polyacrylamide gels and immunoblotted, using specific mono- and polyclonal anti-fibrin(ogen) antibodies. The reduced samples disclosed substantial amounts of high molecular weight material, consistent with α-chain polymers and γγ-dimers, as well as lower molecular weight material, such as α-, β- and γ-chains. No fibrinogen with intact fibrinopeptide A was detectable, and des-AABB fibrin represented a major fibrin derivative in the soluble part of the arterial thrombi. The α-chains were C-terminally degraded, most of them distal to position 259. In conclusion, we have demonstrated the presence of cross-linked fibrin derivatives in soluble, arterial thrombus-related material, without signs of fibrinogen-fibrin hybrids. The fibrin derivatives were C-terminally degraded, thus representing X-oligomeric material, most probably originating from plasmin degradation of insoluble thrombus fibrin. The present study supports the hypothesis of a dynamic equilibrium between clotting and lysis in thrombi. (C) 2000 Lippincott Williams and Wilkins. Chemicals/CAS: Antibodies, Monoclonal; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5; Fibrinopeptide A, 25422-31-5