Print Email Facebook Twitter Physiological impact and context dependency of transcriptional responses: A chemostat study in Saccharomyces cerevisiae Title Physiological impact and context dependency of transcriptional responses: A chemostat study in Saccharomyces cerevisiae Author Tai, S.L. Contributor Pronk, J.T. (promotor) de Winde, J.H. (promotor) Faculty Applied Sciences Date 2007-02-19 Abstract This thesis is a compilation of a four-year PhD project on bakers' yeast (Saccharomyces cerevisiae). Since the entire S. cerevisiae genome sequence became available in 1996, DNA-microarray analysis has become a popular high-information-density tool for analyzing gene expression in this important industrial microorganism and model eukaryote. This thesis explores chemostat-based analysis of the transcriptome (the complete set of messenger RNA molecules) as a tool to understand interaction of S. cerevisiae with its (industrial) environment. Special attention is given to factors that are relevant for beer fermentation. While chemostat-based transcriptome analysis is shown to provide new and important insights into yeast physiology, additional information is required to predict the contribution of genes to cellular fitness. Subject transcriptomicschemostatyeastsaccharomyces cerevisiaegenomicsmicroarraysaffymetrixanaerobiosislow temperatureamino acidfermentation To reference this document use: http://resolver.tudelft.nl/uuid:241801aa-1bcc-4e61-973d-40a0e171fdde Part of collection Institutional Repository Document type doctoral thesis Rights (c) 2007 S.L. Tai Files PDF as_tai_20070219.pdf 1.78 MB Close viewer /islandora/object/uuid:241801aa-1bcc-4e61-973d-40a0e171fdde/datastream/OBJ/view