Print Email Facebook Twitter Coupling Two Ultra-high-Speed Cameras to Elucidate Ultrasound Contrast-Mediated Imaging and Therapy Title Coupling Two Ultra-high-Speed Cameras to Elucidate Ultrasound Contrast-Mediated Imaging and Therapy Author Li, H. (Erasmus MC) Li, X. (TU Delft ImPhys/Medical Imaging) Collado Lara, G. (Erasmus MC) Lattwein, K.R. (Erasmus MC) Mastik, Frits (Erasmus MC) Beurskens, Robert (Erasmus MC) van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC) Verweij, M.A. (TU Delft Technology, Policy and Management; Erasmus MC) de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC) Kooiman, Klazina (Erasmus MC) Faculty Technology, Policy and Management Date 2023 Abstract Ultrasound contrast-mediated medical imaging and therapy both rely on the dynamics of micron- and nanometer-sized ultrasound cavitation nuclei, such as phospholipid-coated microbubbles and phase-change droplets. Ultrasound cavitation nuclei respond non-linearly to ultrasound on a nanosecond time scale that necessitates the use of ultra-high-speed imaging to fully visualize these dynamics in detail. In this study, we developed an ultra-high-speed optical imaging system that can record up to 20 million frames per second (Mfps) by coupling two small-sized, commercially available, 10-Mfps cameras. The timing and reliability of the interleaved cameras needed to achieve 20 Mfps was validated using two synchronized light-emitting diode strobe lights. Once verified, ultrasound-activated microbubble responses were recorded and analyzed. A unique characteristic of this coupled system is its ability to be reconfigured to provide orthogonal observations at 10 Mfps. Acoustic droplet vaporization was imaged from two orthogonal views, by which the 3-D dynamics of the phase transition could be visualized. This optical imaging system provides the temporal resolution and experimental flexibility needed to further elucidate the dynamics of ultrasound cavitation nuclei to potentiate the clinical translation of ultrasound-mediated imaging and therapy developments. Subject CavitationDropletMicrobubbleUltra-high-speed imagingUltrasoundUltrasound contrast agents To reference this document use: http://resolver.tudelft.nl/uuid:27e20e06-0128-4201-aaf6-5f197b33f241 DOI https://doi.org/10.1016/j.ultrasmedbio.2022.08.020 ISSN 0301-5629 Source Ultrasound in Medicine & Biology, 49 (1), 388-397 Part of collection Institutional Repository Document type journal article Rights © 2023 H. Li, X. Li, G. Collado Lara, K.R. Lattwein, Frits Mastik, Robert Beurskens, A.F.W. van der Steen, M.A. Verweij, N. de Jong, Klazina Kooiman Files PDF 1_s2.0_S0301562922005440_main.pdf 1.91 MB Close viewer /islandora/object/uuid:27e20e06-0128-4201-aaf6-5f197b33f241/datastream/OBJ/view