Print Email Facebook Twitter Nuclear Waste and Biocatalysis Title Nuclear Waste and Biocatalysis: A Sustainable Liaison? Author Zhang, W. (TU Delft BT/Biocatalysis; Chinese Academy of Sciences) Liu, H. (TU Delft RST/Applied Radiation & Isotopes; TU Delft RST/Radiation, Science and Technology) van Schie, M.M.C.H. (TU Delft BT/Biocatalysis) Hagedoorn, P.L. (TU Delft BT/Biocatalysis) Alcalde, Miguel (University of the Balearic Islands) Denkova, A.G. (TU Delft RST/Applied Radiation & Isotopes; TU Delft RST/Radiation, Science and Technology) Djanashvili, K. (TU Delft BT/Biocatalysis) Hollmann, F. (TU Delft BT/Biocatalysis) Department RST/Radiation, Science and Technology Date 2020 Abstract It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenases utilize H2O2 as an oxidant to promote highly selective oxyfunctionalization reactions but are also irreversibly inactivated in the presence of too high H2O2 concentrations. Therefore, there is a need for efficient in situ H2O2 generation methods. Here, we show that radiolytic water splitting can be used to promote specific biocatalytic oxyfunctionalization reactions. Parameters influencing the efficiency of the reaction and current limitations are shown. Particularly, oxidative inactivation of the biocatalyst by hydroxyl radicals influences the robustness of the overall reaction. Radical scavengers can alleviate this issue, but eventually, physical separation of the enzymes from the ionizing radiation will be necessary to achieve robust reaction schemes. We demonstrate that nuclear waste can also be used to drive selective, peroxygenase-catalyzed oxyfunctionalization reactions, challenging our view on nuclear waste in terms of sustainability. Subject biocatalysisoxyfunctionalizationperoxygenasesradiationsustainability To reference this document use: http://resolver.tudelft.nl/uuid:8296b91b-4b84-45f1-ac66-98ca370d6282 DOI https://doi.org/10.1021/acscatal.0c03059 ISSN 2155-5435 Source ACS Catalysis, 10 (23), 14195-14200 Part of collection Institutional Repository Document type journal article Rights © 2020 W. Zhang, H. Liu, M.M.C.H. van Schie, P.L. Hagedoorn, Miguel Alcalde, A.G. Denkova, K. Djanashvili, F. Hollmann Files PDF acscatal.0c03059.pdf 1.89 MB Close viewer /islandora/object/uuid:8296b91b-4b84-45f1-ac66-98ca370d6282/datastream/OBJ/view