Print Email Facebook Twitter Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification Title Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification Author Husseini, Abbas Ali (Istanbul Gelisim University) Yazdani, Ali Mohammad (Marmara University; Nisantasi University) Ghadiri, Fatemeh (Istanbul Gelisim University; Istanbul University Cerrahpaşa) Sisman, A.S. (TU Delft Bio-Electronics; Marmara University; Sabanci University) Date 2023 Abstract We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 μL sample volume, glass particle size below 10 μm, concentration of 0.2 μg, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications. Subject cell lysisdropletmicro-glass particlesurface acoustic wave To reference this document use: http://resolver.tudelft.nl/uuid:9f2570f2-c9d3-42fc-a5d8-58777e75234d DOI https://doi.org/10.1002/elsc.202300230 ISSN 1618-0240 Source Engineering in Life Sciences, 24 (1) Part of collection Institutional Repository Document type journal article Rights © 2023 Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, A.S. Sisman Files PDF Engineering_in_Life_Scien ... device.pdf 1.72 MB Close viewer /islandora/object/uuid:9f2570f2-c9d3-42fc-a5d8-58777e75234d/datastream/OBJ/view