Print Email Facebook Twitter Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis Title Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis Author Yayci, Abdulkadir (Ruhr-Universität Bochum) Dirks, Tim (Ruhr-Universität Bochum) Kogelheide, Friederike (Ruhr-Universität Bochum) Alcalde, Miguel (Campus de Cantoblanco) Hollmann, F. (TU Delft BT/Biocatalysis) Awakowicz, Peter (Ruhr-Universität Bochum) Bandow, Julia E. (Ruhr-Universität Bochum) Date 2020 Abstract The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis. Subject atmospheric pressure plasmabiocatalysishydrogen peroxideperoxygenaseplasma chemistry To reference this document use: http://resolver.tudelft.nl/uuid:a28969f9-7546-4fbd-98a1-f58aaa2dca29 DOI https://doi.org/10.1002/cctc.202001225 ISSN 1867-3880 Source ChemCatChem, 12 (23), 5893-5897 Part of collection Institutional Repository Document type journal article Rights © 2020 Abdulkadir Yayci, Tim Dirks, Friederike Kogelheide, Miguel Alcalde, F. Hollmann, Peter Awakowicz, Julia E. Bandow Files PDF cctc.202001225.pdf 1.96 MB Close viewer /islandora/object/uuid:a28969f9-7546-4fbd-98a1-f58aaa2dca29/datastream/OBJ/view