Print Email Facebook Twitter Multiplex genome editing of microorganisms using CRISPR-Cas Title Multiplex genome editing of microorganisms using CRISPR-Cas Author Adiego-Pérez, Belén (Wageningen University & Research) Randazzo, P. (TU Delft BT/Industriele Microbiologie) Daran, J.G. (TU Delft BT/Industriele Microbiologie) Verwaal, René (DSM) Roubos, Johannes A. (DSM) Daran-Lapujade, P.A.S. (TU Delft BT/Industriele Microbiologie) Van Der Oost, John (Wageningen University & Research) Date 2019 Abstract Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing. Subject Cas12aCas9Cell factoriesCRISPR-CasGenome editingMultiplex To reference this document use: http://resolver.tudelft.nl/uuid:a9bb843d-7dbb-4819-b3b4-6ea2d88cbf08 DOI https://doi.org/10.1093/femsle/fnz086 ISSN 0378-1097 Source FEMS Microbiology Letters, 366 (8) Part of collection Institutional Repository Document type review Rights © 2019 Belén Adiego-Pérez, P. Randazzo, J.G. Daran, René Verwaal, Johannes A. Roubos, P.A.S. Daran-Lapujade, John Van Der Oost Files PDF fnz086_1_.pdf 1.46 MB Close viewer /islandora/object/uuid:a9bb843d-7dbb-4819-b3b4-6ea2d88cbf08/datastream/OBJ/view