Print Email Facebook Twitter Towards closed carbon loop fermentations Title Towards closed carbon loop fermentations: Cofeeding of Yarrowia lipolytica with glucose and formic acid Author van Winden, Wouter A. (DSM) Mans, R. (TU Delft BT/Industriele Microbiologie) Breestraat, Stefaan (Bioprocess Pilot Facility) Verlinden, Rob A.J. (Bioprocess Pilot Facility) Mielgo Gomez, A. (TU Delft BT/Industriele Microbiologie) de Hulster, A.F. (TU Delft BT/Industriele Microbiologie) de Bruijn, J.A. (DSM) Noorman, H.J. (TU Delft BT/Bioprocess Engineering; DSM) Date 2022 Abstract A novel fermentation process was developed in which renewable electricity is indirectly used as an energy source in fermentation, synergistically decreasing both the consumption of sugar as a first generation carbon source and emission of the greenhouse gas CO2. As an illustration, a glucose-based process is co-fed with formic acid, which can be generated by capturing CO2 from fermentation offgas followed by electrochemical reduction with renewable electricity. This “closed carbon loop” concept is demonstrated by a case study in which cofeeding formic acid is shown to significantly increase the yield of biomass on glucose of the industrially relevant yeast species Yarrowia lipolytica. First, the optimal feed ratio of formic acid to glucose is established using chemostat cultivations. Subsequently, guided by a dynamic fermentation process model, a fed-batch protocol is developed and demonstrated on laboratory scale. Finally, the developed fed-batch process is tested and proven to be scalable at pilot scale. Extensions of the concept are discussed to apply the concept to anaerobic fermentations, and to recycle the O2 that is co-generated with the formic acid to aerobic fermentation processes for intensification purposes. Subject cofeedingformic acidgreenhouse gas emission reductionscale upYarrowia lipolytica To reference this document use: http://resolver.tudelft.nl/uuid:c9021fb1-31a5-411c-b4bf-fb338dd96f05 DOI https://doi.org/10.1002/bit.28115 ISSN 0006-3592 Source Biotechnology and Bioengineering, 119 (8), 2142-2151 Part of collection Institutional Repository Document type journal article Rights © 2022 Wouter A. van Winden, R. Mans, Stefaan Breestraat, Rob A.J. Verlinden, A. Mielgo Gomez, A.F. de Hulster, J.A. de Bruijn, H.J. Noorman Files PDF Biotech_Bioengineering_20 ... lytica.pdf 2.04 MB Close viewer /islandora/object/uuid:c9021fb1-31a5-411c-b4bf-fb338dd96f05/datastream/OBJ/view