Print Email Facebook Twitter Coupling extracellular glycan composition with metagenomic data in papermill and brewery anaerobic granular sludges Title Coupling extracellular glycan composition with metagenomic data in papermill and brewery anaerobic granular sludges Author Doloman, Anna (Wageningen University & Research) de Bruin, S. (TU Delft BT/Environmental Biotechnology) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology; Aalborg University) Sousa, Diana Z. (Wageningen University & Research; EWUU Alliance) Lin, Y. (TU Delft BT/Environmental Biotechnology) Date 2024 Abstract Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding the microorganisms and biosynthesis pathways responsible for glycan production. In this study, we analysed samples from anaerobic granular sludges treating papermill and brewery wastewater, examining glycans composition and using metagenome-assembled genomes (MAGs) to explore potential biochemical pathways associated with their production. Uronic acids were the predominant constituents of the glycans in extracellular polymeric substances (EPS) produced by the anaerobic granular sludges, comprising up to 60 % of the total polysaccharide content. MAGs affiliated with Anaerolineacae, Methanobacteriaceae and Methanosaetaceae represented the majority of the microbial community (30–50 % of total reads per MAG). Based on the analysis of MAGs, it appears that Anaerolinea sp. and members of the Methanobacteria class are involved in the production of exopolysaccharides within the analysed granular sludges. These findings shed light on the functional roles of microorganisms in glycan production in industrial anaerobic wastewater treatment systems. Subject Anaerobic granular sludgeEPS biosynthesis pathwaysExtracellular polymeric substancesGlycansMetagenomics To reference this document use: http://resolver.tudelft.nl/uuid:e2ef35ae-6901-4f31-b3bc-c21060e49442 DOI https://doi.org/10.1016/j.watres.2024.121240 ISSN 0043-1354 Source Water Research, 252 Part of collection Institutional Repository Document type journal article Rights © 2024 Anna Doloman, S. de Bruin, Mark C.M. van Loosdrecht, Diana Z. Sousa, Y. Lin Files PDF 1-s2.0-S0043135424001404-main.pdf 3.31 MB Close viewer /islandora/object/uuid:e2ef35ae-6901-4f31-b3bc-c21060e49442/datastream/OBJ/view