Print Email Facebook Twitter LEDs for fluorescence microscopy Title LEDs for fluorescence microscopy Author Young, I.T. Garini, Y. Dietrich, H.R.C. Van Oel, W. Liqui Lung, G. Faculty Applied Sciences Department Quantitative Imaging Group Date 2004-07-13 Abstract Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to coherence effects. We are examining the possibility of using the new high-power light-emitting diode (LED) sources as alternatives to the above mentioned sources. LED sources are near monochromatic, are inexpensive, produce little heat, have no coherence problems, have extended lifetimes, are small, and can easily be modulated. We describe experiments comparing various LEDs to other light sources. We compare, for example, a 530 nm LED to the 546 nm line from a mercury lamp on a fluorophore whose absorption maximum is broad and in the middle between these two wavelengths. Subject high-power light-emitting diodesfluorescence microscopymulti-spectral sourcesquantitative microscopy To reference this document use: http://resolver.tudelft.nl/uuid:e3a442cc-4a7c-443b-8344-fcbc1a5b1c25 Publisher SPIE ISSN 0277-786X Source Proceedings of SPIE, 2004 vol. 5324 Part of collection Institutional Repository Document type conference paper Rights (c)2004 Young, I.T., Garini, Y., Dietrich, H.R.C., Van Oel, W., Liqui Lung, G. Files PDF LEDsYoung.pdf 748.62 KB Close viewer /islandora/object/uuid:e3a442cc-4a7c-443b-8344-fcbc1a5b1c25/datastream/OBJ/view