Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

Title

Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

 Author

Xu, X. (TU Delft BT/Biocatalysis)
But, A. (TU Delft BT/Biocatalysis) ORCID 0000-0002-1157-3774
Wever, Ron (Universiteit van Amsterdam)
Hollmann, F. (TU Delft BT/Biocatalysis) ORCID 0000-0003-4821-756X

 Date

2020

 Abstract

The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and kcat of 75 s−1 were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.


 Subject

biocatalysis
glutamic acid
nitriles
oxidative decarboxylation
vanadium chloroperoxidase

 To reference this document use:

http://resolver.tudelft.nl/uuid:e70fc5fc-509f-42ba-9c6f-864f3c6ab4be

 DOI

https://doi.org/10.1002/cctc.201902194

 Embargo date

2020-06-24

 ISSN

1867-3880

 Source

ChemCatChem, 12 (8), 2180-2183

 Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2020 X. Xu, A. But, Ron Wever, F. Hollmann
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