Print Email Facebook Twitter A Programmable Multifunctional 3D Cancer Cell Invasion Micro Platform Title A Programmable Multifunctional 3D Cancer Cell Invasion Micro Platform Author Liu, Q. (TU Delft ChemE/Product and Process Engineering; Guangzhou Laboratory) Muralidharan, A. (TU Delft BN/Stan Brouns Lab) Saateh, A. (TU Delft ChemE/Product and Process Engineering) Ding, Z. (TU Delft Team Kevin Rossi) ten Dijke, Peter (Leiden University Medical Center) Boukany, P. (TU Delft ChemE/Product and Process Engineering) Date 2022 Abstract In the research of cancer cell invasion and metastasis, recreation of physiologically relevant and faithful three-dimensional (3D) tumor models that recapitulate spatial architecture, spatiotemporal control of cell communication and signaling pathways, and integration of extracellular cues remains an open challenge. Here, a programmable multifunctional 3D cancer cell invasion microbuckets-hydrogel (Mb-H) platform is developed by integrating various function-variable microbuckets and extracellular matrix (ECM)-like hydrogels. Based on this Mb-H micro platform, the aggregation of multi-cancer cells is well controlled to form cancer cell spheroids, and the guiding relationship of single-cell migration and collective cell migration during the epithelial-mesenchymal transition (EMT) of cancer cell invasion are demonstrated. By programming and precisely assembling multiple functions in one system, the Mb-H platform with spatial-temporal controlled release of cytokine transforming growth factor beta (TGF-β) and various functionalized Mb-H platforms with intelligent adjustment of cell-matrix interactions are engineered to coordinate the 3D invasive migration of cancer cell spheroids. This programmable and adaptable 3D cancer cell invasion micro platform takes a new step toward mimicking the dynamically changing (localized) tumor microenvironment and exhibits wide potential applications in cancer research, bio-fabrication, cell signaling, and drug screening. Subject 3D cancer cell invasiondirectional cancer cell migrationdynamic TGF-β releasehydrogelsprogrammable multiple functions To reference this document use: http://resolver.tudelft.nl/uuid:ec8a95ae-66a7-4271-be99-3dbe8a0804a8 DOI https://doi.org/10.1002/smll.202107757 ISSN 1613-6810 Source Small (online), 18 (20) Part of collection Institutional Repository Document type journal article Rights © 2022 Q. Liu, A. Muralidharan, A. Saateh, Z. Ding, Peter ten Dijke, P. Boukany Files PDF Small_2022_Liu_A_Programm ... atform.pdf 2.65 MB Close viewer /islandora/object/uuid:ec8a95ae-66a7-4271-be99-3dbe8a0804a8/datastream/OBJ/view