Anaerobic protein degradation

Anaerobic protein degradation: Effects of protein structural complexity, protein concentrations, carbohydrates, and volatile fatty acids

Deng, Z. (TU Delft Sanitary Engineering; Veolia Water Technologies)
Ferreira, Ana Lucia Morgado (Veolia Water Technologies)
Spanjers, H. (TU Delft Sanitary Engineering)
van Lier, J.B. (TU Delft Sanitary Engineering)

2023

Bovine serum albumin (BSA) and casein (CAS) were used in batch tests to compare the protein degradation in the presence and absence of carbohydrates and volatile fatty acids (VFAs). The modified Gompertz model was applied to estimate reaction rates. The results showed that deamination was the rate-limiting step, with a rate ranging between 2.7 and 12.7 mgN·h⁻¹. Higher protein structural complexity negatively affected protein hydrolysis, deamination, and methanogenesis by a factor of 1.6–3.8; whereas a higher protein concentration improved the conversion rates. A carbohydrate:protein COD ratio of 1 improved the hydrolysis rate of BSA from 26 mg·h⁻¹ to 45 mg·h⁻¹, and that of CAS from 98 mg·h⁻¹ to 157 mg·h⁻¹; whereas the deamination rate slightly decreased from 2.7 mg N·h⁻¹ to 2.5 mg N·h⁻¹ and from 6.0 mg N·h⁻¹ to 5.6 mg N·h⁻¹. Additionally, an initial VFAs:protein COD ratio of 1 decreased the CAS deamination rate by 17 %.

Anaerobic digestion
Carbohydrates
Modified Gompertz models
Protein
Rate-limiting step
Volatile fatty acids

http://resolver.tudelft.nl/uuid:39ffcfa9-d77b-4007-be28-073395a0b0ee

https://doi.org/10.1016/j.biteb.2023.101501

2023-12-12

Bioresource Technology Reports, 22

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2023 Z. Deng, Ana Lucia Morgado Ferreira, H. Spanjers, J.B. van Lier