Syntrophy between fermentative and purple phototrophic bacteria to treat and valorize carbohydrate-rich wastewaters

Syntrophy between fermentative and purple phototrophic bacteria to treat and valorize carbohydrate-rich wastewaters

Cerruti, M. (TU Delft BT/Environmental Biotechnology)
Crosset-Perrotin, G. (TU Delft BT/Environmental Biotechnology)
Ananth, Mythili (Student TU Delft)
Rombouts, Julius Laurens (Student TU Delft; Nature’s Principles B.V., Den Haag)
Weissbrodt, D.G. (TU Delft BT/Environmental Biotechnology)

2023

Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor regimes (batch/chemostat) and illumination modes (continuous infrared light, dark, or light/dark cycles) on glucose conversions and process ecology of the interactions between FCB and PPB in mixed cultures. In batch, FCB (>80 % of sequencing read counts) outcompeted PPB, under any light conditions. In chemostat under continuous and alternating irradiance, three FCB populations were enriched (>70 %), while Rhodobacteraceae (PPB) made 30 % of the community. Glucose fermentation products were linked to the dominant FCB. Continuous culturing helped maintaining FCB and PPB in syntrophy: PPB grew on glucose metabolites produced by FCB. Engineering the association between FCB and PPB in mixed-culture processes can help to treat and valorize carbohydrate-rich aqueous waste.

Chemoorganoheterotrophs
Mixed-culture fermentation
Photoorganoheterotrophs
Resource recovery
Syntrophy

http://resolver.tudelft.nl/uuid:41be8047-7942-4ba9-ab4d-361c79cda0cb

https://doi.org/10.1016/j.biteb.2023.101348

Bioresource Technology Reports, 22

Institutional Repository

journal article

© 2023 M. Cerruti, G. Crosset-Perrotin, Mythili Ananth, Julius Laurens Rombouts, D.G. Weissbrodt