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Hoekstra, M. (author), de Weerd, F.A. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The implementation of autotrophic nitrogen removal in the mainstream of a municipal wastewater treatment plant is currently pursued. Among the crucial unknown factors are the kinetic properties of anaerobic ammonium oxidising (anammox) bacteria at low temperatures. In this study we investigated the adaptation of a fast-growing anammox culture...journal article 2017
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van den Berg, E.M. (author), Boleij, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) compete for nitrate in natural and engineered environments. A known important factor in this microbial competition is the ratio of available electron donor and elector acceptor, here expressed as Ac/N ratio (acetate/nitrate-nitrogen). We studied the impact of the Ac/N ratio...journal article 2016
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van Turnhout, A.G. (author), Kleerebezem, R. (author), Heimovaara, T.J. (author)Reliable prediction of the long-term behavior of environmental systems such as Municipal Solid Waste (MSW) landfills is challenging. While many driving forces influence this behavior, characterization of them is limited by measurement techniques. Therefore, a model structure for reliable prediction needs to optimally combine all measured...journal article 2016
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Marang, L. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author)The process for non-axenic polyhydroxyalkanoate (PHA) production from organic waste generally comprises three steps: acidogenic fermentation of the waste stream, enrichment of a PHA-producing culture, and production of the PHA. This study assesses the feasibility of combining the enrichment and production step. Harvesting PHA-rich biomass...journal article 2016
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Junicke, H. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)Many anaerobic conversions proceed close to thermodynamic equilibrium and the microbial groups involved need to share their low energy budget to survive at the thermodynamic boundary of life. This study aimed to investigate the kinetic and thermodynamic control mechanisms of the electron transfer during syntrophic butyrate conversion in non...journal article 2015
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Kleerebezem, R. (author), Joosse, B. (author), Rozendaal, R. (author), Van Loosdrecht, M.C.M. (author)Anaerobic digestion for the production of methane containing biogas is the classic example of a resource recovery process that combines stabilization of particulate organic matter or wastewater treatment with the production of a valuable end-product. Attractive features of the process include the production of a single end-product from a...journal article 2015
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Mooij, P.R. (author), De Jongh, L.D. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)Microalgae have the potential to supply a biobased society with essential feedstocks like sugar and lipids. Besides being productive, strains used for this purpose should grow fast, be resistant to predators, and have good harvestability properties. Diatoms, a class of siliceous algae, have these and other preferred characteristics. In this...journal article 2015
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Pronk, M. (author), Abbas, B. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)The influence of sludge age on granular sludge formation and microbial population dynamics in a methanol- and acetate-fed aerobic granular sludge system operated at 35°C was investigated. During anaerobic feeding of the reactor, methanol was initially converted to methane by methylotrophic methanogens. These methanogens were able to withstand...journal article 2015
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Van Turnhout, A.G. (author), Kleerebezem, R. (author), Heimovaara, T.J. (author)In this study, we aim to validate the reaction network with an idealized experiment. We want to show that 1) rate controlling parameters are identifiable from the measured data by inverse modeling, and 2) that this network is able to predict the measured emissions in the experiment given the initial conditions. In the experiment, cabbage is used...conference paper 2015
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Van Turnhout, A.G. (author), Brandstatter, C. (author), Kleerebezem, R. (author), Heimovaara, T.J. (author)The aim of our study is to investigate previously mentioned aspects with scenario analyses via a mechanistic biogeochemical reaction network model that contains the emission controlling reactions. In order to find such a network, we apply the gray modeling toolbox developed by van Turnhout et al. (2015) on a measured dataset used in the study of...conference paper 2015
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Mooij, P.R. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)The invention is directed to a method for producing an enriched diatom culture, comprising subjecting a starting culture comprising one or more diatom species to selective pressure, thus giving a competitive advantage to storage compound producing species of diatoms, by subjecting said starting culture, under non-limiting bioavailable silicon...patent 2015
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Tamis, J. (author), Sorokin, D.Y. (author), Jiang, Y. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)Background Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil...journal article 2015
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Jiang, Y. (author), Mikova, G. (author), Kleerebezem, R. (author), Van der Wielen, L.A.M. (author), Cuellar Soares, M.C. (author)This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS (0.2 w/v% SDS) could reach 99% purity, with more...journal article 2015
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Effect and behaviour of different substrates in relation to the formation of aerobic granular sludgePronk, M. (author), Abbas, B. (author), Al-zuhairy, S.H.K. (author), Kraan, R. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)When aerobic granular sludge is applied for industrial wastewater treatment, different soluble substrates can be present. For stable granular sludge formation on volatile fatty acids (e.g. acetate), production of storage polymers under anaerobic feeding conditions has been shown to be important. This prevents direct aerobic growth on readily...journal article 2015
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- Van Turnhout, A.G. (author), Heimovaara, T.J. (author), Kleerebezem, R. (author) conference paper 2014
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Junicke, H. (author), Abbas, B. (author), Oentoro, J. (author), Van Loosdrecht, M. (author), Kleerebezem, R. (author)Identification of individual biomass concentrations is a crucial step towards an improved understanding of anaerobic digestion processes and mixed microbial conversions in general. The knowledge of individual biomass concentrations allows for the calculation of biomass specific conversion rates which form the basis of anaerobic digestion models....journal article 2014
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Van Turnhout, A.G. (author), Heimovaara, T.J. (author), Kleerebezem, R. (author)In this paper we present an approach to describe organic degradation within immobile water regions of Municipal Solid Waste (MSW) landfills which is best described by the term “gray box” model. We use a simplified set of dominant (bio)chemical and physical reactions and realistic environmental conditions. All equations, relationships and...conference paper 2013
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- Van Turnhout, A.G. (author), Heimovaara, T.J. (author), Kleerebezem, R. (author) conference paper 2013
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Hu, Z. (author), Lotti, T. (author), De Kreuk, B.M. (author), Kleerebezem, R. (author), Van Loosdrecht, M. (author), Kruit, J. (author), Jetten, M.S.M. (author), Kartala, B. (author)Currently, nitritation-anammox (anaerobic ammonium oxidation) bioreactors are designed to treat wastewaters with high ammonium concentrations at mesophilic temperatures (25 to 40°C). The implementation of this technology at ambient temperatures for nitrogen removal from municipal wastewater following carbon removal may lead to more-sustainable...journal article 2013
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Method for obtaining an open phototrophic culture with improved storage compound production capacityTamis, J. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)The invention is directed to a method for producing an open phototrophic culture with improved storage compound production capability. In accordance with the invention, a starting culture is submitted to selective pressure, thus giving a competitive advantage to storage compound producing species, by subjecting said starting culture to a cycle...patent 2013