Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium

Background Staphylococcus aureus is a common human and animal opportunistic pathogen. In humans nasal carriage of S. aureus is a risk factor for various infections. Methicillin-resistant S. aureus ST398 is highly prevalent in pigs in Europe and North America. The mechanism of successful pig colonization by MRSA ST398 is poorly understood....

The cessation of continuous turbulence as precursor of the very stable nocturnal boundary layer

The mechanism behind the collapse of turbulence in the evening as a precursor to the onset of the very stable boundary layer is investigated. To this end a cooled, pressure-driven flow is investigated by means of a local similarity model. Simulations reveal a temporary collapse of turbulence whenever the surface heat extraction, expressed in its...

The minimum wind speed for sustainable turbulence in the nocturnal boundary layer

The collapse of turbulence in the nocturnal boundary layer is studied by means of a simple bulk model that describes the basic physical interactions in the surface energy balance. It is shown that for a given mechanical forcing, the amount of turbulent heat that can be transported downward is limited to a certain maximum. In the case of weak...

High-performance simulations of turbulent clouds on a desktop PC: Exploiting the GPU

Convective boundary layers driven by nonstationary surface heat fluxes

In this study the response of dry convective boundary layers to nonstationary surface heat fluxes is systematically investigated. This is relevant not only during sunset and sunrise but also, for example, when clouds modulate incoming solar radiation. Because the time scale of the associated change in surface heat fluxes may differ from case to...

Reply to the Comments by J. C. Bergmann on the Paper: “Local Similarity in the Stable Boundary Layer and Mixing Length Approaches: Consistency of Concepts

Formulation of the Dutch Atmospheric Large-Eddy Simulation (DALES) and overview of its applications

The current version of the Dutch Atmospheric Large-Eddy Simulation (DALES) is presented. DALES is a large-eddy simulation code designed for studies of the physics of the atmospheric boundary layer, including convective and stable boundary layers as well as cloudy boundary layers. In addition, DALES can be used for studies of more specific cases,...

A statistical approach to the life cycle analysis of cumulus clouds selected in a virtual reality environment

In this study, a new method is developed to investigate the entire life cycle of shallow cumuli in large eddy simulations. Although trained observers have no problem in distinguishing the different life stages of a cloud, this process proves difficult to automate, because cloud-splitting and cloud-merging events complicate the distinction...

Local Similarity in the Stable Boundary Layer and Mixing-Length Approaches: Consistency of Concepts

In stably stratified flows vertical movement of eddies is limited by the fact that kinetic energy is converted into potential energy, leading to a buoyancy displacement scale z B . Our new mixing-length concept for turbulent transport in the stable boundary layer follows a rigid-wall analogy, in the sense that we assume that the buoyancy length...

A refined view of vertical mass transport by cumulus convection

The purpose of this letter is to show that the traditional view of transport by shallow cumulus clouds needs important refinement. On the basis of a straightforward geometrical analysis of Large Eddy Simulation results of shallow cumulus clouds, we conclude (1) that the upward mass transport by clouds is strongly dominated by regions close to...

Wind and boundary layers in Rayleigh-Bénard convection. II: Boundary layer character and scaling

Wind and boundary layers in Rayleigh-Bénard convection. I: Analysis and modeling

Spectral analysis of boundary layers in Rayleigh-Bénard convection

Suitability of quantum cascade laser spectroscopy for CH4 and N2O eddy covariance flux measurements

A quantum cascade laser spectrometer was evaluated for eddy covariance flux measurements of CH4 and N2O using three months of continuous measurements at a field site. The required criteria for eddy covariance flux measurements including continuity, sampling frequency, precision and stationarity were examined. The system operated continuously at...

Incompressibility of the Leray-? model for wall-bounded flows

This study shows that the Leray-? model does not explicitly enforce a divergence-free field for the filtered velocity. While this condition is automatically satisfied in the absence of boundaries, bounded domains require extra attention. It is shown, both analytically and through simulations of Rayleigh–Bénard convection, that incompressibility...

Identification of the wind in Rayleigh–Bénard convection

Using a symmetry-accounting ensemble-averaging method, we have identified the wind in unbounded Rayleigh–Bénard convection. This makes it possible to distinguish the wind from fluctuations and to identify dynamic features of each. We present some results from processing five independent three-dimensional direct numerical simulations of a ? = 4...