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Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacteriumsaccharolyticum

Zheng, T. (author), Olson, D.G. (author), Tian, L. (author), Bomble, Y.J. (author), Himmel, M.E. (author), Lo, J. (author), Hon, S. (author), Shaw, A.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. Although engineered strains of T. saccharolyticum produce ethanol with a yield of 90% of the theoretical maximum, engineered strains of...

journal article 2015

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The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading

Holwerda, E.K. (author), Thorne, P.G. (author), Olson, D.G. (author), Amador-Noguez, D. (author), Engle, N.L. (author), Tschaplinksi, T.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Background Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ?10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which...

journal article 2014

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Atypical Glycolysis in Clostridium thermocellum

Zhou, J. (author), Olson, D.G. (author), Argyros, D.A. (author), Deng, Y. (author), Van Gulik, W.M. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)

Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity....

journal article 2013

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Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...

journal article 2008

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Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of L-Arabinose

Wisselink, H.W. (author), Toirkens, M.J. (author), Del Rosario Franco Berriel, M. (author), Winkler, A.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as L-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production,...

journal article 2007

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Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

Metabolic engineering of Saccharomyces cerevisiae for ethanol production from d-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment d-xylose, the ketoisomer d-xylulose can be metabolised slowly. Conversion of d-xylose into d-xylulose is...

journal article 2007

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Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: Current status

Van Maris, A.J.A. (author), Abbott, D.A. (author), Bellissimi, E. (author), Van den Brink, J. (author), Kuyper, M. (author), Lutik, M.A.H. (author), Wisselink, H.W. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 2006

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Effects of oxygen limitation on sugar metabolism in yeasts: A continuous-culture study of the Kluyver effect

Weusthuis, R.A. (author), Visser, W. (author), Pronk, J.T. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1994

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Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation

Verduyn, C. (author), Postma, E. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1992

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Production and localization of beta-fructosidase in asynchronous and synchronous chemostat cultures of yeasts

Rouwenhorst, R.J. (author), van der Baan, A.A. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1991

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A theoretical evaluation of growth yields of yeasts

Verduyn, C. (author), Stouthamer, A.H. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

Growth yields of Saccharomyces cerevisiae and Candida utilis in carbon-limited chemostat cultures were evaluated. The yields on ethanol and acetate were much lower in S. cerevisiae, in line with earlier reports that site I phosphorylation is absent in this yeast. However, during aerobic growth on glucose both organisms had the same cell yield....

journal article 1991

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Localization of inulinase and invertase in Kluyveromyces species

Rouwenhorst, R.J. (author), Ritmeester, W.S. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1990

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Transient-State Analysis of Metabolic Fluxes in Crabtree-Positive and Crabtree-Negative Yeasts

Van Urk, H. (author), Voll, W.S. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1990

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Glucose transport in Crabtree-positive and Crabtree-negative yeasts

Van Urk, H. (author), Postma, E. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1989

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Localization and kinetics of pyruvate-metabolizing enzymes in relation to aerobic alcoholic fermentation in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621

Van Urk, H. (author), Schipper, D. (author), Breddveld, G.J. (author), Mak, P.R. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1989

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Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae

Postma, E. (author), Verduyn, C. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1989

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Preparation of D-xylulose from D-xylose: Enzyme and Microbial Technology

Pronk, J.T. (author), Bakker, A.W. (author), Van Dam, H.E. (author), Straathof, A.J.J. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1988

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Production, Distribution, and Kinetic Properties of Inulinase in Continuous Cultures of Kluyveromyces marxianus CBS 6556

Rouwenhorst, R.J. (author), Visser, L.E. (author), Van Der Baan, A.A. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1988

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Alcoholic fermentation by 'non-fermentative' yeasts

Van Dijken, J.P. (author), Van den Bosch, E. (author), Hermans, J.J. (author), Rodrigues de Miranda, L. (author), Scheffers, W.A. (author)

journal article 1986

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Redox balances in the metabolism of sugars by yeasts. (NAD(H); NADP(H); glucose metabolism; xylose fermentation; ethanol; Crabtree effect; Custers effect)

Van Dijken, J.P. (author), Scheffers, W.A. (author)

journal article 1986