The archaeal Cdv cell division system

The Cdv system is the protein machinery that performs cell division and other membrane-deforming processes in a subset of archaea. Evolutionarily, the system is closely related to the eukaryotic ESCRT machinery, with which it shares many structural and functional similarities. Since its first description 15 years ago, the understanding of the...

Looking Back To Move Forward: Studying the Ancient Archaeal Cdv Cell Division Machinery for Synthetic Cells

All living organisms share the need to replicate and proliferate to ensure the survival of their species. In prokaryotes, this is generally guaranteed by a process of cell division where a mother cell is split into two equally sized daughter cells, and it is a complex and heterogeneous process across all the different species. When looking into...

The archaeal division protein CdvB1 assembles into polymers that are depolymerized by CdvC

The Cdv proteins constitute the cell division system of the Crenarchaea, a machinery closely related to the ESCRT system of eukaryotes. Using a combination of TEM imaging and biochemical assays, we here present an in vitro study of Metallosphaera sedula CdvB1, the Cdv protein that is believed to play a major role in the constricting ring that...

Biosynthesis of protein filaments for the creation of a minimal cell

Humanity has achieved to decipher the most fundamental mechanics of cellular life. Nevertheless, despite intense efforts there are still considerable gaps in our understanding of cellular processes. Traditionally, biologists investigate life by observation of existing lifeforms. In order to assign functions to biological components, it is common...