Intracellular product recycling in high succinic acid producing yeast at low pH

Background: The metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, the product transport can become bidirectional, i.e. the acid is reentering the cell and is again exported or even...

Towards high-quality multi-spot-welded joints in thermoplastic composite structures

Ultrasonic welding is a promising assembly technique for thermoplastic composites and it is well-suited for spot welding. In this study, two typical welding process control strategies, i.e. displacement-controlled and energy-controlled welding, were selected to manufacture spot-welded joints. The influence of different boundary conditions,...

An extended probabilistic method for reliability analysis under mixed aleatory and epistemic uncertainties with flexible intervals

The reliability analysis approach based on combined probability and evidence theory is studied in this paper to address the reliability analysis problem involving both aleatory uncertainties and epistemic uncertainties with flexible intervals (the interval bounds are either fixed or variable as functions of other independent variables). In...

Aircraft Disposal and Recycle Cost Estimation

The present study develops a method for the sake of evaluating Disposal and Recycle (D&R) cost in view of the increasing demand in aircraft retirement. Firstly, a process model is extracted. The subordinated cost elements are also identified. Next, the cost aggregations based on the D&R process steps are discussed. Moreover, it proposes...

Comparative analysis of in-plane and out-of-plane mechanical behaviour of spot-welded and mechaincally fastened joints in thermoplastic composites

Ultrasonic welding is a promising assembly technique for thermoplastic composites and it is well-suited for spot welding. In this paper, the in-plane and out-of-plane mechanical behaviour of ultrasonically spot-welded and mechanically fastened joints are investigated by double-lap shear and pull-through tests, respectively. Special attention was...

Cytosolic NADPH balancing in Penicillium chrysogenum cultivated on mixtures of glucose and ethanol

The in vivo flux through the oxidative branch of the pentose phosphate pathway (oxPPP) in Penicillium chrysogenum was determined during growth in glucose/ethanol carbon-limited chemostat cultures, at the same growth rate. Non-stationary 13C flux analysis was used to measure the oxPPP flux. A nearly constant oxPPP flux was found for all glucose...