Detecting structural heterogeneity in single-molecule localization microscopy data

<br/>For decades, the resolution of fluorescent light microscopy has been bounded by Abbe’s diffraction limit to λ/2NA. Super-resolution methods, awarded with the 2014 Nobel Prize in Chemistry, use tricks to overcome this limit. The general idea is to image blinking fluorophores for a multitude of frames, such that each frame only contains a...