Fluidic Force Microscopy and Atomic Force Microscopy Unveil New Insights into the Interactions of Preosteoblasts with 3D-Printed Submicron Patterns

Physical patterns represent potential surface cues for promoting osteogenic differentiation of stem cells and improving osseointegration of orthopedic implants. Understanding the early cell–surface interactions and their effects on late cellular functions is essential for a rational design of such topographies, yet still elusive. In this work...

Mechanical characterization of nanopillars by atomic force microscopy

Micro- and nano-patterns are gaining increasing attraction in several fields ranging from nanoelectronics to bioengineering. The mechanical properties of the nanostructures (nanopillars, nanotubes, nanowires, etc.) are highly relevant for many applications but challenging to determine. Existing mechanical characterization methods require...

Experimental setup for dynamic analysis of microand nano-mechanical systems in vacuum, gas, and liquid

An experimental setup to perform dynamic analysis of a micro- and nano-mechanical system in vacuum, gas, and liquid is presented. The setup mainly consists of a piezoelectric excitation part and the chamber that can be either evacuated for vacuum, or filled with gas or water. The design of the piezoelectric actuator was based on a Langevin...

A model for controlled dosing of femto-litre volume liquids using hollow microcantilever

A hollow microcantilever is used instead of a conventional microcantilever to dispense and aspirate liquids in the femto-litre (10^-15 L) volume range in an atomic force microscope (AFM) setup. The inherent force sensing capability of the cantilever is used to monitor the fluid manipulation in-situ. At this small scale, parameters...

In situ Stiffness adjustment of AFM probes by two orders of magnitude

The choice on which type of cantilever to use for Atomic Force Microscopy (AFM) depends on the type of the experiment being done. Typically, the cantilever has to be exchanged when a different stiffness is required and the entire alignment has to be repeated. In the present work, a method to adjust the stiffness in situ of a commercial AFM...

Nano-Workbench: A Combined Hollow AFM Cantilever and Robotic Manipulator

To manipulate liquid matter at the nanometer scale, we have developed a robotic assembly equipped with a hollow atomic force microscope (AFM) cantilever that can handle femtolitre volumes of liquid. The assembly consists of four independent robots, each sugar cube sized with four degrees of freedom. All robots are placed on a single platform...

Scanning Probe Microscope-Based Fluid Dispensing

Advances in micro and nano fabrication technologies have enabled fabrication of smaller and more sensitive devices for applications not only in solid-state physics but also in medicine and biology. The demand for devices that can precisely transport material, specifically fluids are continuously increasing. Therefore, integration of various...