Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies

Bacterial cells require DNA segregation machinery to properly distribute a genome to both daughter cells upon division. The most common system involved in chromosome and plasmid segregation in bacteria is the ParABS system. A core protein of this system - partition protein B (ParB) - regulates chromosome organization and chromosome...

Performance analysis of an idealized Darrieus–Savonius combined vertical axis wind turbine

To investigate the effect of force distributions of each turbine component on the power performance of the Darrieus–Savonius combined vertical axis wind turbine (hybrid VAWT), the hybrid VAWT is modeled as idealized turbine under various force distributions. The goal of idealization is to simplify the intricate interactions between the...

The Fluid Dynamic Basis for Actuator Disc and Rotor Theories: Revised Second Edition

The first rotor performance predictions were published by Joukowsky exactly 100 years ago. Although a century of research has expanded the knowledge of rotor aerodynamics enormously, and modern computer power and measurement techniques now enable detailed analyses that were previously out of reach, the concepts proposed by Froude, Betz,...

In situ Stiffness adjustment of AFM probes by two orders of magnitude

The choice on which type of cantilever to use for Atomic Force Microscopy (AFM) depends on the type of the experiment being done. Typically, the cantilever has to be exchanged when a different stiffness is required and the entire alignment has to be repeated. In the present work, a method to adjust the stiffness in situ of a commercial AFM...

Nano-Workbench: A Combined Hollow AFM Cantilever and Robotic Manipulator

To manipulate liquid matter at the nanometer scale, we have developed a robotic assembly equipped with a hollow atomic force microscope (AFM) cantilever that can handle femtolitre volumes of liquid. The assembly consists of four independent robots, each sugar cube sized with four degrees of freedom. All robots are placed on a single platform...

Scanning Probe Microscope-Based Fluid Dispensing

Advances in micro and nano fabrication technologies have enabled fabrication of smaller and more sensitive devices for applications not only in solid-state physics but also in medicine and biology. The demand for devices that can precisely transport material, specifically fluids are continuously increasing. Therefore, integration of various...

Evaluation of EMG, force and joystick as control interfaces for active arm supports

Background: The performance capabilities and limitations of control interfaces for the operation of active movement-assistive devices remain unclear. Selecting an optimal interface for an application requires a thorough understanding of the performance of multiple control interfaces. Methods: In this study the performance of EMG-, force- and...

Optical trapping at low numerical aperture

A theory of optical trapping at low Numerical Aperture (NA) is presented. The theory offers an analytical description of the competition between the stabilizing gradient and destabilizing scattering force. The trade-off can be characterized by a single dimensionless trapping parameter, which increases with bead size to wavelength ratio ?/?, m,...