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Microstructural characterization of crack-healing enabled by bacteria-embedded polylactic acid (PLA) capsules

He, S. (author), Wan, Z. (author), Chen, Y. (author), Jonkers, H.M. (author), Schlangen, E. (author)

The current study investigates short-term and long-term crack-healing behaviour of mortars embedded with bacteria-based poly-lactic acid (PLA) capsules under both ideal and realistic environmental conditions. Two sets of specimens were prepared and subjected to different healing regimes, with the first set kept in a mist room for varying...

journal article 2023

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Assessment of Functional Performance, Self-Healing Properties and Degradation Resistance of Poly-Lactic Acid and Polyhydroxyalkanoates Composites

Rossi, E. (author), Raghavan, A. (author), Copuroglu, Oguzhan (author), Jonkers, H.M. (author)

In this study, the applicability of two bacteria-based healing agents (e.g., poly-lactic acid and polyhydroxyalkanoate) in blast furnace slag cement (BFSC) mortar has been assessed. An experimental campaign on the functional properties, self-healing capacity, freezing–thawing and carbonation resistance has been conducted in comparison with plain...

journal article 2022

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Influence of self-healing induced by polylactic-acid and alkanoates-derivates precursors on transport properties and chloride penetration resistance of sound and cracked mortar specimens

Rossi, E. (author), Rahul, R. (author), Copuroglu, Oguzhan (author), Jonkers, H.M. (author)

The possible beneficial impact of self-healing on chloride transport through cracks has been assessed for two bacteria-based self-healing mortar mixtures in comparison with Ordinary Portland cement mortar. Intact self-healing specimens featured lower chloride transport coefficients thanks to a denser microstructure and to the formation of a...

journal article 2022

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From waste to self-healing concrete: A proof-of-concept of a new application for polyhydroxyalkanoate

Vermeer, C.M. (author), Rossi, E. (author), Tamis, J. (author), Jonkers, H.M. (author), Kleerebezem, R. (author)

Polyhydroxyalkanoate (PHA) production is a promising opportunity to recover organic carbon from waste streams. However, widespread application of waste-derived PHA as biodegradable plastic is restricted by expensive purification steps, high quality requirements, and a fierce competition with the conventional plastic market. To overcome these...

journal article 2021

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Assessment of the self-healing capacity of cementitious materials through active thin sections

Rossi, E. (author), Romero Rodriguez, C. (author), Jonkers, H.M. (author), Copuroglu, Oguzhan (author)

Since self-healing of cementitious materials can theoretically improve the service-life of concrete structures, it has gathered significant attention from both researchers and industry during the last two decades. Many researchers have proposed different methods to assess and quantify the self-healing capacity (i.e. the ability of...

journal article 2021

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Self-healing capacity of mortars with added-in bio-plastic bacteria-based agents: Characterization and quantification through micro-scale techniques

Roy, Rahul (author), Rossi, E. (author), Silfwerbrand, Johan (author), Jonkers, H.M. (author)

In this study, a novel non-toxic, biodegradable bacteria-based healing agent known as alkanoate derivatives (AKD) derived from wastewater was investigated for its self-healing efficiency of the mortar specimens in comparison to the already-developed healing agents made of lactic acid derivatives (PLA). Mortar with different percentages of...

journal article 2021

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On the Applicability of a Precursor Derived from Organic Waste Streams for Bacteria-Based Self-Healing Concrete

Rossi, E. (author), Vermeer, C.M. (author), Mors, R.M. (author), Kleerebezem, R. (author), Copuroglu, Oguzhan (author), Jonkers, H.M. (author)

Bacteria-based self-healing concrete has the ability to heal cracks due to the bacterial conversion of incorporated organic compounds into calcium carbonate. Precipitates seal the cracks, theoretically increasing the service life of constructions. The aim of this paper is to propose a precursor for bacteria-based self-healing concrete derived...

journal article 2021

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Bacteria‐based self‐healing concrete: Evaluation of full scale demonstrator projects

Mors, R.M. (author), Jonkers, H.M. (author)

Bacteria‐based self‐healing concrete is an innovative concrete that contains a self‐healing agent that provides the material with enhanced autonomous crack‐sealing performance. A specific type of this concrete, based on a healing agent composed of bacterial spores and lactate as carbon source, has been developed and applied by the Delft...

journal article 2020

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Bio-Based self-healing mortar

Md Yunus, Balqis (author), Schlangen, E. (author), Jonkers, H.M. (author)

This paper investigates the effects of alkaliphilic spore-forming bacteria of the genus Bacillus on the compressive strength of the mortar cube and the healing capacity of the bacteria as healing agent on mortar containing crack. The experiments were carried out using cube test, stereomicroscopy and environmental scanning electron microscopy ...

journal article 2019

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Volume fraction, thickness, and permeability of the sealing layer in microbial self-healing concrete containing biogranules

Ersan, Yusuf Cagatay (author), Palin, D. (author), Yengec Tasdemir, Sena Busra (author), Tasdemir, Kasim (author), Jonkers, H.M. (author), Boon, Nico (author), De Belie, Nele (author)

Autonomous repair systems in construction materials have become a promising alternative to current unsustainable and labor-intensive maintenance methods. Biomineralization is a popular route that has been applied to enhance the self-healing capacity of concrete. Various axenic microbial cultures were coupled with protective carriers, and their...

journal article 2018

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Selection of Nutrient Used in Biogenic Healing Agent for Cementitious Materials

Tziviloglou, E. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)

Biogenic self-healing cementitious materials target on the closure of micro-cracks with precipitated inorganic minerals originating from bacterial metabolic activity. Dormant bacterial spores and organic mineral compounds often constitute a biogenic healing agent. The current paper focuses on the investigation of the most appropriate organic...

journal article 2017

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Effect on Concrete Surface Water Absorption upon Addition of Lactate Derived Agent

Mors, R.M. (author), Jonkers, H.M. (author)

Water tightness of a concrete cover layer is important, as it is typically used as a protective coating of the steel reinforcement. Water tightness can be impaired by crack formation or by permeability. A bacteria-based lactate-derived healing agent (HA) can be added to concrete to enhance the potential for restoration of water tightness....

journal article 2017

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The Use of Alkaliphilic Bacteria-based Repair Solution for Porous Network Concrete Healing Mechanism

Sangadji, S. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)

Bacteria induced calcium carbonate precipitation based on metabolic conversion of nutrients has been acknowledged for having potentials in self-healing cement-based materials. Recent studies have shown the development of bacteria-based repair solution (liquid) for concrete surface repair. This article demonstrates the feasible application of...

conference paper 2017

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Reduction of water permeation through cracks in mortar by addition of bacteria based healing agent

Mors, R.M. (author), Jonkers, H.M. (author)

conference paper 2015

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Preparation and optimization of bio-based and light weight aggregate-based healing agent for application in concrete

Tziviloglou, E. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)

The innovative technology of self-healing concrete allows the material to repair the open micro-cracks that can endanger the structure’s durability, due to ingress of aggressive liquids. Various concepts of self-healing concrete use encapsulation techniques, in order to immobilize and protect the healing agent during mixing and setting. In this...

conference paper 2015

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Field application of self-healing concrete with natural fibres as linings for irrigation canals in Ecuador

Sierra Beltran, M.G. (author), Jonkers, H.M. (author), Mors, R.M. (author), Mera-Ortiz, W. (author)

This paper describes the first field application of self-healing concrete with alkaliphilic spore-forming bacteria and reinforced with natural fibres. The application took place in the highlands in Ecuador in July 2014. The concrete was cast as linings for an irrigation canal that transports water from glaciers in the Andean mountains to...

conference paper 2015

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Geopolymer Coating of Bacteria-containing Granules for Use in Self-healing Concrete

De Koster, S.A.L. (author), Mors, R.M. (author), Nugteren, H.W. (author), Jonkers, H.M. (author), Meesters, G.M.H. (author), Van Ommen, J.R. (author)

Concrete structures are often reinforced with steel. In order for the reinforcement to take over tensile forces, concrete has to crack. Through such cracks, water and compounds that are harmful to concrete can enter. This can cause durability issues like leakage, concrete degradation and reinforcement corrosion. In situ repair of cracks is often...

journal article 2015

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Bacteria-based self-healing concrete to increase durability of structures

Tziviloglou, E. (author), Jonkers, H.M. (author), Schlangen, E. (author)

conference paper 2014

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Practical approach production of bacteria-based agent-contained light weight aggregates to make concrete self-healing

Mors, R.M. (author), Jonkers, H.M. (author)

A functional experimental concrete system has been developed in our lab, in which a two component bacteria-based healing agent contained in a protective reservoir is included in the concrete mixture. Incorporated bacteria have the potential to produce copious amounts of calcium carbonate based crystals from supplied mineral precursor compounds....

conference paper 2013

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Microbially mediated carbonation of marine alkaline minerals: Potential for concrete crack healing

Jonkers, H.M. (author), Palin, D. (author), Flink, P. (author), Thijssen, A. (author)

Concrete constructions in the marine environment suffer from chemical attack of sea salts which can induce damage to both the concrete matrix and embedded steel reinforcement. For example, ingress of sulfate and chloride ions can respectively result in detrimental ettringite formation and enhanced corrosion of the steel rebars. The first...

conference paper 2013