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Slijkhuis, Nuria (author), Razzi, F. (author), Korteland, Suze Anne (author), Heijs, Bram (author), van Gaalen, Kim (author), Duncker, Dirk J. (author), van der Steen, A.F.W. (author), van Steijn, V. (author), van Beusekom, Heleen M.M. (author), van Soest, G. (author)Coronary atherosclerosis is caused by plaque build-up, with lipids playing a pivotal role in its progression. However, lipid composition and distribution within coronary atherosclerosis remain unknown. This study aims to characterize lipids and investigate differences in lipid composition across disease stages to aid in the understanding of...journal article 2024
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Szabo Corbacho, M. (author), Sharma, Pragnya (author), Míguez, Diana (author), de la Sovera, Victoria (author), Brdjanovic, Damir (author), Etchebehere, Claudia (author), Garcia, H. (author), van Lier, J.B. (author)The study investigated the effects of long-chain fatty acids (LCFA) on anaerobic sludge treating lipid-rich wastewater. It involved batch experiments with three sludge samples: two acclimated to lipids and one non-acclimated. The experiments aimed to observe the degradation of LCFA, specifically oleate and palmitate, by dosing them at...journal article 2024
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Yang, X. (author), Shin, D. (author), Yu, Z. (author), Watanabe, Kenji (author), Taniguchi, Takashi (author), Babenko, Vitaliy (author), Hofmann, Stephan (author), Caneva, S. (author)Fluorescence imaging is an invaluable tool to investigate biomolecular dynamics, mechanics, and interactions in aqueous environments. Two-dimensional materials offer large-area, atomically smooth surfaces for wide-field biomolecule imaging. Despite the success of graphene for on-chip biosensing and biomolecule manipulation, its strong...journal article 2024
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Policastro, G. (author), Ebrahimi, S. (author), Weissbrodt, D.G. (author), Fabbricino, Massimiliano (author), van Loosdrecht, Mark C.M. (author)A dual-growth-limited continuous operated bioreactor (chemostat) was used to enhance lipid accumulation in an enrichment culture of microalgae. The light intensity and nitrogen concentration where both limiting factors resulting in high lipid accumulation in the mixed culture. Both conditions of light and nitrogen excess and deficiency were...journal article 2024
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Spiekhout, Sander (author), van Elburg, Benjamin (author), Voorneveld, J.D. (author), de Jong, N. (author), Versluis, Michel (author), Bosch, Johannes G. (author), Segers, Tim (author)Phospholipid-coated microbubbles with a uniform acoustic response are a promising avenue for functional ultrasound sensing. A uniform acoustic response requires both a monodisperse size distribution and uniform viscoelastic shell properties. Monodisperse microbubbles can be produced in a microfluidic flow focusing device. Here, we investigate...journal article 2024
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de Juana Fabra, Amalia (author)Atherosclerosis is an arterial condition characterized by the accumulation mainly of lipid tissue, resulting in the narrowing and stiffening of arteries. It is the leading cause of death among cardiovascular diseases and its development has been associated with wall shear (WSS) and wall mechanical stress (WMS). This study aimed to analyse WSS...master thesis 2023
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Esselman, Allison B. (author), Patterson, Nathan Heath (author), Migas, L.G. (author), Dufresne, Martin (author), Djambazova, Katerina V. (author), Colley, Madeline E. (author), Van de Plas, Raf (author), Spraggins, Jeffrey M. (author)The glomerulus is a multicellular functional tissue unit (FTU) of the nephron that is responsible for blood filtration. Each glomerulus contains multiple substructures and cell types that are crucial for their function. To understand normal aging and disease in kidneys, methods for high spatial resolution molecular imaging within these FTUs...journal article 2023
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Van de Cauter, L. (author), van Buren, L. (author), Koenderink, G.H. (author), Ganzinger, Kristina A. (author)Creating an artificial cell from the bottom up is a long-standing challenge and, while significant progress has been made, the full realization of this goal remains elusive. Arguably, one of the biggest hurdles that researchers are facing now is the assembly of different modules of cell function inside a single container. Giant unilamellar...review 2023
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Kuttippurath, Vivek (author), Slijkhuis, Nuria (author), Liu, Shengnan (author), van Soest, G. (author)Significance: Spectroscopic analysis of optical coherence tomography (OCT) data can yield added information about the sample's chemical composition, along with high-resolution images. Typical commercial OCT systems operate at wavelengths that may not be optimal for identifying lipid-containing samples based on absorption features. Aim: The...journal article 2023
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Burghoorn, Niels (author)Medical technology has seen great progress over the last centuries, developing increasingly more specialised therapy to benefit human health. The rise of the pharmaceutical industry and the research it drives are contributors to this success. However, in humanity's attempt to tackle more and more complex health issues, the conventional methods...master thesis 2022
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Bosma, Douwe (author)An isogeometric finite element method for incompressible fluid film equations is presented. The method can be applied to numerically model the behaviour of thin cellular membranes, such as lipid bilayers. The membranes are represented by infinitely thin closed surfaces. Both the surface parametrization and analysis are based on state-of-the-art...master thesis 2022
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de Ridder, Djim (author)In animal cells, cell shape is primarily regulated by the actin cortex, a thin filament network connected to the plasma membrane. The architecture of the cortex is considered a key regulator of its function. Visualising the cortex is difficult due to the high density of numerous small-sized proteins involved in its formation. Consequently, the...master thesis 2022
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Argiz, Lucía (author), Correa-Galeote, David (author), Val del Río, Ángeles (author), Mosquera-Corral, Anuska (author), Gonzalez Cabaleiro, R. (author)The lipid fraction of the effluents generated in several food-processing activities can be transformed into polyhydroxyalkanoates (PHAs) and triacylglycerides (TAGs), through open culture biotechnologies. Although competition between storing and non-storing populations in mixed microbial cultures (MMCs) has been widely studied, the right...journal article 2022
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Rivera, Emilio S. (author), Weiss, Andy (author), Migas, L.G. (author), Freiberg, Jeffrey A. (author), Djambazova, Katerina V. (author), Neumann, Elizabeth K. (author), Van de Plas, Raf (author), Spraggins, Jeffrey M. (author), Skaar, Eric P. (author), Caprioli, Richard M. (author)Introduction: Although Staphylococcus aureus is the leading cause of biofilm-related infections, the lipidomic distributions within these biofilms is poorly understood. Here, lipidomic mapping of S. aureus biofilm cross-sections was performed to investigate heterogeneity between horizontal biofilm layers. Methods: S. aureus biofilms were...journal article 2022
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Amador, G.J. (author), van Dijk, Dennis (author), Kieffer, R.Y. (author), Aubin-Tam, M.E. (author), Tam, D.S.W. (author)Vital biological processes, such as trafficking, sensing, and motility, are facilitated by cellular lipid membranes, which interact mechanically with surrounding fluids. Such lipid membranes are only a few nanometers thick and composed of a liquid crystalline structure known as the lipid bilayer. Here, we introduce an active, noncontact, two...journal article 2021
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Prabudiansyah, I. (author), van der Valk, R.A. (author), Aubin-Tam, M.E. (author)FtsH is a membrane-bound protease that plays a crucial role in proteolytic regulation of many cellular functions. It is universally conserved in bacteria and responsible for the degradation of misfolded or misassembled proteins. A recent study has determined the structure of bacterial FtsH in detergent micelles. To properly study the function...journal article 2021
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Losch, M.S. (author), Swamy, A. (author), Elmi-Terander, Adrian (author), Edström, Erik (author), Hendriks, B.H.W. (author), Dankelman, J. (author)Background: The increased popularity of minimally invasive spinal surgery calls for a revision of guidance techniques to prevent injuries of nearby neural and vascular structures. Lipid content has previously been proposed as a distinguishing criterion for different bone tissues to provide guidance along the interface of cancellous and...journal article 2021
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Langeveld, Simone A.G. (author), Beekers, Inés (author), Collado Lara, G. (author), van der Steen, A.F.W. (author), de Jong, N. (author), Kooiman, Klazina (author)Phospholipid-coated microbubbles are ultrasound contrast agents that can be employed for ultrasound molecular imaging and drug delivery. For safe and effective implementation, microbubbles must respond uniformly and predictably to ultrasound. Therefore, we investigated how lipid handling and phase distribution affected the variability in the...journal article 2021
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Kattan, J.M. (author), Doerr, A. (author), Dogterom, A.M. (author), Danelon, C.J.A. (author)Genetic control over a cytoskeletal network inside lipid vesicles offers a potential route to controlled shape changes and DNA segregation in synthetic cell biology. Bacterial microtubules (bMTs) are protein filaments found in bacteria of the genus Prosthecobacter. They are formed by the tubulins BtubA and BtubB, which polymerize in the...journal article 2021
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Ahn, Anne Catherine (author), Jongepier, Evelien (author), Merijn Schuurmans, J. (author), Irene, W. (author), Sinninghe Damsté, Jaap S. (author), Galinski, Erwin A. (author), Roman, Pawel (author), Sorokin, Dimitry Y. (author), Muyzer, Gerard (author)The genus Thioalkalivibrio comprises sulfur-oxidizing bacteria thriving in soda lakes at high pH and salinity. Depending on the geographical location and the season, these lakes can strongly vary in temperature. To obtain a comprehensive understanding of the molecular and physiological adaptations to low temperature, we compared the responses...journal article 2021