Modulation enhanced multi-emitter localization microscopy: Multi-emitter fitting with structured illumination patterns for high imaging density super-resolution localization microscopy

For a long time, scientists believed that it is not possible to achieve a better microscopic resolution than half the wavelength of light (~ 200 nm). During the last decades, various super-resolution fluorescence microscopy techniques have been developed, offering high-resolution imaging beyond the diffraction limit. It has been established as...

Analyzing single molecule emission patterns using Deep Learning

The time taken to generate a super-resolution image and the quality of the final synthetic image depends on the performance of the localization algorithm which is used in the localization microscopy pipeline. The most precise and accurate algorithms are mostly iterative and they take a long time to generate the localization list while the faster...

Data-Driven PSF Modeling using B-Splines

Super Resolution Microscopy is a technique to image objects with resolution higher than the diffraction limit, the fundamental limit to the resolution of a microscope. One of the techniques used for Super Resolution Microscopy is Single Molecule Localization Microscopy (SMLM). For this method, a sample is labeled using fluorophores (fluorescent...