Analyzing point spread functions for multiple emitter fitting by posterior density estimation

The microscope is an essential tool for biologists. Since the late 16th century, it has given researchers a better understanding of cell processes and greatly advanced healthcare. In this century, Single molecule localization microscopy (SMLM) has revolutionized optical microscopy by breaking the optical diffraction limit. Sparsely activating...

Data-Driven PSF Modeling using B-Splines

Super Resolution Microscopy is a technique to image objects with resolution higher than the diffraction limit, the fundamental limit to the resolution of a microscope. One of the techniques used for Super Resolution Microscopy is Single Molecule Localization Microscopy (SMLM). For this method, a sample is labeled using fluorophores (fluorescent...