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Kievits, A.J. (author), Duinkerken, B. H.Peter (author), Fermie, Job (author), Lane, R. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Recent advances in electron microscopy techniques have led to a significant scale up in volumetric imaging of biological tissue. The throughput of electron microscopes, however, remains a limiting factor for the volume that can be imaged in high resolution within reasonable time. Faster detection methods will improve throughput. Here, we have...journal article 2024
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Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...journal article 2021