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van Veldhuizen, B.N. (author), Zera, E. (author), van Biert, L. (author), Modena, S. (author), Visser, K. (author), Aravind, P.V. (author)Solid Oxide Fuel Cell (SOFC) systems have the potential to reduce emissions from seagoing vessels. However, it is unknown whether ship motions influence the system's operation. In this research, a 1.5 kW SOFC module is operated on an inclination platform that emulates ship motions, to evaluate the influence of static and dynamic inclinations...journal article 2023
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Schwab, Andrea (author), Wesdorp, Marinus A. (author), Xu, J. (author), Abinzano, Florencia (author), Loebel, Claudia (author), Levato, Riccardo (author), Eglin, David (author), Narcisi, Roberto (author), Malda, Jos (author), van Osch, G.J.V.M. (author)Background: The use of acellular hydrogels to repair osteochondral defects requires cells to first invade the biomaterial and then to deposit extracellular matrix for tissue regeneration. Due to the diverse physicochemical properties of engineered hydrogels, the specific properties that allow or even improve the behaviour of cells are not yet...journal article 2023
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Wehrens, Martijn (author), Krah, Laurens H.J. (author), Towbin, Benjamin D. (author), Hermsen, Rutger (author), Tans, S.J. (author)The inherent stochasticity of metabolism raises a critical question for understanding homeostasis: are cellular processes regulated in response to internal fluctuations? Here, we show that, in E. coli cells under constant external conditions, catabolic enzyme expression continuously responds to metabolic fluctuations. The underlying...journal article 2023
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Zhao, Y. (author), Datta, Kunal (author), Paggiaro, Giulia (author), Liu, Hanchen (author), Fardousi, Mohua (author), Santbergen, R. (author), Procel Moya, P.A. (author), Han, C. (author), Yang, G. (author), Weeber, A.W. (author), Zeman, M. (author), Mazzarella, L. (author), Isabella, O. (author)Monolithic perovskite/c-Si tandem solar cells have attracted enormous research attention and have achieved efficiencies above 30%. This work describes the development of monolithic tandem solar cells based on silicon heterojunction (SHJ) bottom- and perovskite top-cells and highlights light management techniques assisted by optical simulation...journal article 2023
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Li, L. (author), Lukszo, Z. (author), Schmitz, J.E.J. (author)Hydrogen is becoming increasingly popular as a clean, secure, and affordable energy source for the future. This study develops an approach for designing a PV–battery–electrolyzer–fuel cell energy system that utilizes hydrogen as a long-term storage medium and battery as a short-term storage medium. The system is designed to supply load demand...journal article 2023
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Zhang, Yuxuan (author), Zhai, Xiaoqiang (author), Zhang, Fengyuan (author), Zhang, Zhongbin (author), Hooman, K. (author), Zhang, Hai (author), Wang, Xiaolin (author)Enhancing gas-liquid mass transfer is key to promote gas hydrate formation kinetics. Encapsulation of CO<sub>2</sub> hydrate is expected to dramatically increase gas-liquid contact to enhance mass transfer. However, gas hydrate encapsulation has never been proposed as the technical issues of gas permeation through capsule shells have never...journal article 2023
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Ördög, Balázs (author), De Coster, Tim (author), Dekker, Sven O. (author), Bart, Cindy I. (author), Zhang, Juan (author), Boink, Gerard J.J. (author), Bax, Wilhelmina H. (author), Deng, Shanliang (author), den Ouden, B.L. (author), de Vries, Antoine A.F. (author), Pijnappels, Daniël A. (author)To unlock new research possibilities by acquiring control of action potential (AP) morphologies in excitable cells, we developed an opto-electronic feedback loop-based system integrating cellular electrophysiology, real-time computing, and optogenetic approaches and applied it to monolayers of heart muscle cells. This allowed accurate...journal article 2023
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Blöbaum, Luisa (author), Haringa, C. (author), Grünberger, Alexander (author)Bioprocesses are scaled up for the production of large product quantities. With larger fermenter volumes, mixing becomes increasingly inefficient and environmental gradients get more prominent than in smaller scales. Environmental gradients have an impact on the microorganism's metabolism, which makes the prediction of large-scale performance...review 2023
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Godino, E. (author), Danelon, C.J.A. (author)Mimicking bacterial cell division in well-defined cell-free systems has the potential to elucidate the minimal set of proteins required for cytoskeletal formation, membrane constriction, and final abscission. Membrane-anchored FtsZ polymers are often regarded as a sufficient system to realize this chain of events. By using purified FtsZ and...journal article 2023
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Abdelhakim, Ahmed (author), Soeiro, Thiago B. (author), Stecca, M. (author), Canales, Francisco (author)Compact and efficient power converter solutions are seen to be the backbone of future transportation systems in order to cope with the ongoing transition toward greener systems. Such systems usually comprise a main load section, in which one or more propulsion or traction motors are connected, in addition to an auxiliary load, which might...journal article 2023
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Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...journal article 2023
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Li, Wenhuai (author), Li, Mengran (author), Guo, Y. (author), Hu, Zhiwei (author), Zhou, Chuan (author), Brand, Helen E.A. (author), Peterson, Vanessa K. (author), Pao, Chih Wen (author), Chen, Chien Te (author)Oxygen-ion conducting perovskite oxides are important functional materials for solid oxide fuel cells and oxygen-permeable membranes operating at high temperatures (>500 °C). Co-doped perovskites have recently shown their potential to boost oxygen-related kinetics, but challenges remain in understanding the underlying mechanisms. This...journal article 2023
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Polat, S. (author), Kortlever, R. (author), Eral, H.B. (author)Electrochemical carbon dioxide (CO<sub>2</sub>) reduction is a promising route to convert intermittent renewable energy into fuels and valuable chemical products. Separation of CO<sub>2</sub> reduction products by ion-selective electrochemical technology may play a decisive role in the pursuit of commercially viable CO<sub>2</sub> reduction...journal article 2023
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Liu, H. (author), Nadar, R.A. (author), Fauzia, R.P. (author), Laan, A.C. (author), Wang, R. (author), van Cooten, Quenteijn (author), Carroll, E.C.M. (author), Eelkema, R. (author), Denkova, A.G. (author)Combination of therapies is a common strategy in cancer treatment. Such combined therapies only have merit provided that there is superior therapeutic outcome with fewer side effects, compared to single therapies. Here, this work explores the possibility to combine chemotherapy with radionuclide therapy using polymeric micelles as a delivery...journal article 2023
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Abil, Z. (author), Restrepo Sierra, A.M. (author), Danelon, C.J.A. (author)In cell-free gene expression, low input DNA concentration severely limits the phenotypic output, which may impair in vitro protein evolution efforts. We address this challenge by developing CADGE, a strategy that is based on clonal isothermal amplification of a linear gene-encoding dsDNA template by the minimal Φ29 replication machinery and...journal article 2023
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da Silva, Renan F. (author), Hansen, Jeff E. (author), Lowe, Ryan J. (author), Rijnsdorp, D.P. (author), Buckley, Mark L. (author)Nearshore rocky reefs with scales of order 10–100 m are common along the world's coastline and often shape wave-driven hydrodynamics and shoreline morphology in their lee. The interaction of waves with these reefs generally results in either two or four-cell mean circulation systems (2CC and 4CC, respectively), with diverging flows behind the...journal article 2023
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Ji, Encheng (author), Leijsten, Lieke (author), Witte-Bouma, Janneke (author), Rouchon, Adelin (author), Di Maggio, Nunzia (author), Banfi, Andrea (author), van Osch, G.J.V.M. (author), Farrell, Eric (author), Lolli, Andrea (author)Tissue engineering bone via endochondral ossification requires the generation of a cartilage template which undergoes vascularisation and remodelling. While this is a promising route for bone repair, achieving effective cartilage vascularisation remains a challenge. Here, we investigated how mineralisation of tissue-engineered cartilage...journal article 2023
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Stoter, Stein K.F. (author), Divi, Sai C. (author), van Brummelen, E. Harald (author), Larson, Mats G. (author), de Prenter, Frits (author), Verhoosel, Clemens V. (author)In this article, we study the effect of small-cut elements on the critical time-step size in an immersogeometric explicit dynamics context. We analyze different formulations for second-order (membrane) and fourth-order (shell-type) equations, and derive scaling relations between the critical time-step size and the cut-element size for various...journal article 2023
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Geelhoed, Jeanine S. (author), Thorup, Casper A. (author), Bjerg, Jesper J. (author), Schreiber, Lars (author), Nielsen, Lars Peter (author), Schramm, Andreas (author), Meysman, F.J.R. (author), Marshall, Ian P.G. (author)Bacterial cells can vary greatly in size, from a few hundred nanometers to hundreds of micrometers in diameter. Filamentous cable bacteria also display substantial size differences, with filament diameters ranging from 0.4 to 8 µm. We analyzed the genomes of cable bacterium filaments from 11 coastal environments of which the resulting 23 new...journal article 2023
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Cordes, Martijn (author), Pike-Overzet, K. (author), van den Akker, E.B. (author), Staal, F.J.T. (author), Canté-Barrett, Kirsten (author)Traditionally, flow cytometry has been the preferred method to characterize immune cells at the single-cell level. Flow cytometry is used in immunology mostly to measure the expression of identifying markers on the cell surface, but—with good antibodies—can also be used to assess the expression of intracellular proteins. The advent of single...review 2023