- document
-
Langsdorf, Alexander (author), Volkmar, Marianne (author), Ulber, Roland (author), Hollmann, F. (author), Holtmann, Dirk (author)Green waste, especially of municipal origin, is currently used as a material only to a limited extent. However, the large material flows could also be used in a more economical way if they were integrated into biorefinery concepts. Besides the production of basic and fine chemicals, green waste could also be used as source of industrial...journal article 2023
- document
-
Wu, Bin (author), Wang, Xiangyun (author), Yang, Bo (author), Ma, Yunjian (author), Hollmann, F. (author), Wang, Yonghua (author)In the present work we propose a bienzymatic cascade for the oxyfunctionalisation of cycloalkanes to cyclic alcohols/cyclic ketones. By combining a H<sub>2</sub>O<sub>2</sub>-dependent peroxygenase with a O<sub>2</sub>-consuming and H<sub>2</sub>O<sub>2</sub>-producing alcohol oxidase an overall aerobic oxidation system was established. A...journal article 2023
- document
-
Wu, Y. (author), Paul, C.E. (author), Hollmann, F. (author)This review article critically compares two widely used types of catalysis, chemo- and biocatalysis, and provides insights on their greenness according to specified parameters. A comparative analysis of the environmental impact of chemo- and biocatalytic oxyfunctionalisation reactions based on published experimental data reveals that both...journal article 2023
- document
-
De Santos, Patricia Gomez (author), Mateljak, Ivan (author), Hoang, Manh Dat (author), Fleishman, Sarel J. (author), Hollmann, F. (author), Alcalde, Miguel (author)The generation of enantiodivergent biocatalysts for C-H oxyfunctionalizations is ever more important in modern synthetic chemistry. Here, we have applied the FuncLib algorithm based on phylogenetic and Rosetta calculations to design a diverse repertoire of active, stable, and enantiodivergent fungal peroxygenases. 24 designs, each carrying 4...journal article 2023
- document
-
Gomez de Santos, Patricia (author), González-Benjumea, Alejandro (author), Fernandez-Garcia, Angela (author), Aranda, Carmen (author), Wu, Y. (author), But, A. (author), Molina-Espeja, Patricia (author), Zhang, W. (author), Hollmann, F. (author)The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. In this study, we have engineered a highly regioselective fungal peroxygenase for the ω-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near...journal article 2023
- document
-
Xu, X. (author), Hilberath, T. (author), Hollmann, F. (author)Heme-dependent oxygenases (i.e. P450 monooxygenases and peroxygenases) are highly selective catalysts for the selective oxyfunctionalisation or organic compounds. Both enzyme classes exhibit mechanistic similarities (i.e. using so-called compound I (CpdI) as active oxidation species) and differences in how CpdI is formed. From the differences...review 2023
- document
- Reetz, Manfred T. (author), Hollmann, F. (author) journal article 2023
- document
-
Holtmann, Dirk (author), Hollmann, F. (author), Bouchaut, B.F.H.J. (author)In September 2015, the United Nations General Assembly established the 2030 Agenda for Sustainable Development, which includes 17 Sustainable Development Goals (SDGs) [...].journal article 2023
- document
-
Ma, Yunjian (author), Zhong, Xuanru (author), Wu, Bin (author), Lan, Dongming (author), Zhang, Hao (author), Hollmann, F. (author), Wang, Yonghua (author)Hydrocarbons are essential base chemicals as energy carriers and starting materials for chemical manufacture. So-called fatty acid photodecarboxylases (FAPs) represent interesting catalysts for the conversion of natural fatty acids into hydrocarbons thereby giving access to alkanes from renewable feedstock. Today, however, only few FAPs are...journal article 2023
- document
-
Ma, Yunjian (author), Li, Zongquan (author), Zhang, Hao (author), Wong, Vincent Kam Wai (author), Hollmann, F. (author), Wang, Yonghua (author)Aromatic aldehydes are important aromatic compounds for the flavour and fragrance industry. In this study, a parallel cascade combining aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) and unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO) to convert aromatic primary alcohols into high-value aromatic aldehydes is...journal article 2023
- document
-
Li, Huanhuan (author), Shen, Qianqian (author), Zhou, Xiaoying (author), Duan, Peigao (author), Hollmann, F. (author), Huang, Yawen (author), Zhang, Wuyuan (author)Chiral sulfoxides are valuable building blocks in asymmetric synthesis. However, the biocatalytic synthesis of chiral sulfoxides is still challenged by low product titres. Herein, we report the use of peroxygenase as a catalyst for asymmetric sulfoxidation under non-aqueous conditions. Upon covalent immobilisation, the peroxygenase showed...journal article 2023
- document
-
Burek, Bastien O. (author), Dawood, A.W.H. (author), Hollmann, F. (author), Liese, Andreas (author), Holtmann, Dirk (author)Enzyme catalysis, made tremendous progress over the last years in identification of new enzymes and new enzymatic reactivity’s as well as optimization of existing enzymes. However, the performance of the resulting processes is often still limited, e.g., in regard of productivity, realized product concentrations and the stability of the enzymes....journal article 2022
- document
-
Silva de Miranda, Amanda (author), Milagre, C.D.D.F. (author), Hollmann, F. (author)Alcohol dehydrogenases (ADHs) have become important catalysts for stereoselective oxidation and reduction reactions of alcohols, aldehydes and ketones. The aim of this contribution is to provide the reader with a timely update on the state-of-the-art of ADH-catalysis. Mechanistic basics are presented together with practical information about the...journal article 2022
- document
-
Hilberath, T. (author), van Troost, A.G.L. (author), Alcalde, Miguel (author), Hollmann, F. (author)The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard to the type and concentration of the respective co-solvent. In this contribution, we investigated...journal article 2022
- document
- Holtmann, Dirk (author), Hollmann, F. (author) journal article 2022
- document
-
Bouchaut, B.F.H.J. (author), Hollmann, F. (author), Asveld, L. (author)The increasing societal demand for safer, biobased products, and processes<br/>creates opportunities for industrial biotechnology and chemistry. To succeed,<br/>controlled learning about new emerging risks is crucial but both fields endure<br/>difficulty in doing so by their respective regulation and risk management culture.journal article 2022
- document
-
Puchľová, Eva (author), Hilberath, T. (author), Vranková, Kvetoslava (author), Hollmann, F. (author)Non-enantioselective alcohol dehydrogenases (ADHs) are rarely found in the biocatalysis portfolio. Generally, highly enantioselective ADHs are sought for. Using such ADHs for the oxidation of racemic alcohols generally results in a kinetic resolution of the starting material, which is unfavourable if the ketone represents the product of interest...journal article 2022
- document
-
Reeve, Holly A. (author), Nicholson, Jake (author), Altaf, Farieha (author), Lonsdale, Thomas H. (author), Preissler, Janina (author), Lauterbach, Lars (author), Lenz, Oliver (author), Leimkühler, Silke (author), Hollmann, F. (author), Paul, C.E. (author), Vincent, Kylie A. (author)We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50%...journal article 2022
- document
-
Hanefeld, U. (author), Hollmann, F. (author), Paul, C.E. (author)Biocatalysis has an enormous impact on chemical synthesis. The waves in which biocatalysis has developed, and in doing so changed our perception of what organic chemistry is, were reviewed 20 and 10 years ago. Here we review the consequences of these waves of development. Nowadays, hydrolases are widely used on an industrial scale for the...review 2022
- document
-
Chen, Shaohang (author), Zhang, Jiaan (author), Zeng, Zhigang (author), Dai, Zongjie (author), Wang, Qinhong (author), Wever, Ron (author), Hollmann, F. (author), Zhang, W. (author)A chemoenzymatic method for the synthesis of haloethers is presented. A combination of enzymatic hypohalite synthesis with spontaneous oxidation of alkenes and nucleophilic attack by various alcohols enabled the synthesis of a wide range of haloethers. The reaction system has been characterised and current imitations have been worked out. In...journal article 2022