Re-scan confocal microscopy

Scanning twice for better resolution

Journal article (2013)
Department
IST/Imaging Science and Technology (AS) (TU Delft)
Copyright: © 2013 Optical Society of America
DOI: https://doi.org/doi:10.1364/BOE.4.002644
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Published Date

25-10-2013

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Source:

Biomedical Optics Express, 4 (11), 2013

Faculty

Applied Sciences

Department

IST/Imaging Science and Technology

Abstract

We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.