Physiological impact and context dependency of transcriptional responses

A chemostat study in Saccharomyces cerevisiae

Doctoral thesis (2007)

Authors

S.L. Tai

Contributors

J.T. Pronk (promotor)

J.H. de Winde (promotor)

Faculty

Applied Sciences () (TU Delft)

Yeast Transcriptomics Chemostat Saccharomyces cerevisiae Genomics Microarrays Affymetrix Anaerobiosis Low temperature Amino acid Fermentation

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Published Date

19-02-2007

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Faculty

Applied Sciences

Abstract

This thesis is a compilation of a four-year PhD project on bakers' yeast (Saccharomyces cerevisiae). Since the entire S. cerevisiae genome sequence became available in 1996, DNA-microarray analysis has become a popular high-information-density tool for analyzing gene expression in this important industrial microorganism and model eukaryote. This thesis explores chemostat-based analysis of the transcriptome (the complete set of messenger RNA molecules) as a tool to understand interaction of S. cerevisiae with its (industrial) environment. Special attention is given to factors that are relevant for beer fermentation. While chemostat-based transcriptome analysis is shown to provide new and important insights into yeast physiology, additional information is required to predict the contribution of genes to cellular fitness.

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