Conformation and Dynamics of DNA Confined in Slitlike Nanofluidic Channels

Journal article (2008)
Department
Kavli Institute of Nanoscience Delft (AS) (TU Delft)
Copyright: © 2008 Bonthuis, D.J. ; Meyer, C. ; Stein, D. ; Dekker, C. ; American Physical Society
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Published Date

05-09-2008

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Source:

Physical Review Letters, 101 (10), 2008

Faculty

Applied Sciences

Department

Kavli Institute of Nanoscience Delft

Abstract

Using laser fluorescence microscopy, we study the shape and dynamics of individual DNA molecules in slitlike nanochannels confined to a fraction of their bulk radius of gyration. With a confinement size spanning 2 orders of magnitude, we observe a transition from the de Gennes regime to the Odijk regime in the scaling of both the radius of gyration and the relaxation time. The radius of gyration and the relaxation time follow the predicted scaling in the de Gennes regime, while, unexpectedly, the relaxation time shows a sharp decrease in the Odijk regime. The radius of gyration remains constant in the Odijk regime. Additionally, we report the first measurements of the effect of confinement on the shape anisotropy.

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