Title
Serpentinimonas gen. Nov., serpentinimonas raichei sp. nov., serpentinimonas barnesii sp. nov. and serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs
Author
Bird, Lina J. (Naval Research Laboratory; University of Southern California)
Kuenen, J.G. (TU Delft BT/Environmental Biotechnology; University of Southern California) 
Osburn, Magdalena R. (Northwestern University)
Tomioka, Naotaka (Japan Agency for Marine-Earth Science and Technologies (JAMSTEC))
Ishii, Shun’Ichi (Japan Agency for Marine-Earth Science and Technologies (JAMSTEC); Institute for Extra-cutting- edge Science and Technology Avant-garde Research (X-star))
Barr, Casey (University of Southern California)
Nealson, Kenneth H. (University of Southern California)
Suzuki, Shino (Japan Agency for Marine-Earth Science and Technologies (JAMSTEC); Institute of Space and Astronautical Science (ISAS)/JAXA; Institute for Extra-cutting- edge Science and Technology Avant-garde Research (X-star))
Date
2021
Abstract
Three highly alkaliphilic bacterial strains designated as A1T, H1T and B1T were isolated from two highly alkaline springs at The Cedars, a terrestrial serpentinizing site. Cells from all strains were motile, Gram-negative and rod-shaped. Strains A1T, H1T and B1T were mesophilic (optimum, 30 °C), highly alkaliphilic (optimum, pH 11) and facultatively autotrophic. Major cellular fatty acids were saturated and monounsaturated hexadecenoic and octadecanoic acids. The genome size of strains A1T, H1T and B1T was 2574013, 2475906 and 2623236 bp, and the G+C content was 66.0, 66.2 and 66.1mol%, respectively. Analysis of the 16S rRNA genes showed the highest similarity to the genera Malikia (95.1–96.4%), Macromonas (93.0–93.6%) and Hydrogenophaga (93.0–96.6%) in the family Comamonadaceae. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis based on core gene sequences revealed that the isolated strains diverged from the related species, forming a distinct branch. Average amino acid identity values of strains A1T, H1T and B1T against the genomes of related members in this family were below 67%, which is below the suggested threshold for genera boundaries. Average nucleotide identity by blast values and digital DNA– DNA hybridization among the three strains were below 92.0 and 46.6% respectively, which are below the suggested thresholds for species boundaries. Based on phylogenetic, genomic and phenotypic characterization, we propose Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov. (type strain A1T=NBRC 111848T=DSM 103917T), Serpentinimonas barnesii sp. nov. (type strain H1T= NBRC 111849T=DSM 103920T) and Serpentinimonas maccroryi sp. nov. (type strain B1T=NBRC 111850T=DSM 103919T) belonging to the family Comamonadaceae. We have designated Serpentinimonas raichei the type species for the genus because it is the dominant species in The Cedars springs.
Subject
Alkaliphile
Autotrophic growth
Serpentinization
Serpentinomonas/Serpentinimonas
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http://resolver.tudelft.nl/uuid:a2e0de1e-eca2-4b8c-9bd9-bc7e8425704d
DOI
https://doi.org/10.1099/ijsem.0.004945
ISSN
1466-5026
Source
International Journal of Systematic and Evolutionary Microbiology, 71 (8)
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2021 Lina J. Bird, J.G. Kuenen, Magdalena R. Osburn, Naotaka Tomioka, Shun’Ichi Ishii, Casey Barr, Kenneth H. Nealson, Shino Suzuki