Development towards a robust low-cost Fourier Ptychographic microscope

For the detection of malaria parasites

Master thesis (2019)

Authors

A.N.M. Heemels Mechanical Engineering

Contributors

T.E. Agbana Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering (coach)
Gleb Vdovin Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering (mentor)
S.F. Pereira ImPhys/Optics - AS (graduation committee member)
Jan-Carel Diehl Design for Sustainability - IDE (graduation committee member)
J.C. Diehl Design for Sustainability - IDE (graduation committee member)
Jan Carel Diehl Design for Sustainability - IDE (graduation committee member)
Faculty
Mechanical Engineering, Mechanical Engineering
Copyright: © 2019 Alex Heemels
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Published Date

03-09-2019

Language

English

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Faculty

Mechanical Engineering

Abstract

This thesis discusses developments towards a low-cost Fourier Ptychographic microscope for label free imaging of malaria parasites.
A review of the morphology and life cycle of malaria and the main diagnostic methods for its detection is followed by an introduction to Fourier Ptychography with emphasis on the underlying imaging principles and phase retrieval algorithms which are at the core of the algorithms used.
The practical realization of the Fourier Ptychographic setup with the required resolution has proven to be very challenging due to its susceptibility to errors when operating the system at its theoretical limits.
Insights from in-depth analyses of the effects of quantization noise, intensity drop-off due to angled illumination, and partial coherence are presented. These insights rule in- or out these potential error sources and help identify potential mitigations in the design.
In the final chapters the realization of the setup is described, and the results with real blood smear samples are used to illustrate the interference of the error sources. The thesis concludes with considerations for further research and recommendations for international collaboration.

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MscThesisAlexHeemels.pdf
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