A Molecular Basis for Tungstate Selectivity in Prokaryotic ABC Transport Systems

Journal article (2011)
Department
Biotechnology (AS) (TU Delft)
Copyright: © 2011 American Society for Microbiology
DOI: https://doi.org/doi:10.1128/JB.05056-11
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Published Date

30-09-2011

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Source:

JOURNAL OF BACTERIOLOGY, Sept. 2011, p. 4999–5001

Faculty

Applied Sciences

Department

Biotechnology

Abstract

The essential trace compounds tungstate and molybdate are taken up by cells via ABC transporters. Despite their similar ionic radii and chemical properties, the WtpA protein selectively binds tungstate in the presence of molybdate. Using site-directed mutagenesis of conserved binding pocket residues, we established a molecular basis for tungstate selectivity.