Title
Sandwich-like heterostructured nanomaterials immobilized laccase for the degradation of phenolic pollutants and boosted enzyme stability
Author
Li, Mengyu (Zhengzhou University)
Bai, Yahan (Zhengzhou University)
Zhuang, Wei (Zhengzhou University; Nanjing Tech University)
Liu, Jinle (Zhengzhou University)
Wang, Zhi (Zhengzhou University)
Rao, Yuan (Zhengzhou University)
Li, Mengran (TU Delft ChemE/Materials for Energy Conversion and Storage)
Ying, Hanjie (Nanjing Tech University)
Ouyang, Pingkai (Nanjing Tech University)
Date
2023
Abstract
A novel magnetic 2D/2D heterogeneous structure MXene@NiFe-LDH@Fe3O4 was prepared for immobilization of laccase. In this work, two-dimensional MXene nanosheets with abundant surface functional groups were heterogeneously assembled with layered double hydroxide (LDH) by in situ co-precipitation method, and magnetic nanoparticle Fe3O4 with excellent biocompatibility and rapid separation of materials and substrates was introduced subsequently, and then silane coupling agent was coated on the surface of MXene@NiFe-LDH@Fe3O4. The functionalized MXene@NiFe-LDH@Fe3O4 was employed as a carrier to immobilize laccase from Trametes-Versicolor. The enzyme loading of the nanocomposite material is as high as 167.9 mg/g. Compared with free enzymes, the immobilized laccase showed a notable improvement in stability in a wider range of pHs (2.0–8.0), temperatures (25–60 °C), and organic solvent concentration (1–5 M). The reusability study suggested that after 7 cycles of repeated catalysis, the degradation efficiency could reach 55.5% for 2,4-dichlorophenol, 92.1% for bisphenol A and70.9% for pyrocatechol. The results provide a new carrier preparation strategy for the efficient immobilization of laccase.
Subject
2D nanomaterials
Heterogeneous assembly
Immobilized laccase
Simulated industrial wastewater
To reference this document use:
http://resolver.tudelft.nl/uuid:c487fe21-f61e-4544-ad12-5fe656e48d2a
DOI
https://doi.org/10.1016/j.colsurfa.2022.130820
Embargo date
2023-07-01
ISSN
0927-7757
Source
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 660
Bibliographical note
Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2023 Mengyu Li, Yahan Bai, Wei Zhuang, Jinle Liu, Zhi Wang, Yuan Rao, Mengran Li, Hanjie Ying, Pingkai Ouyang