Print Email Facebook Twitter The contribution of lipid peroxidation to membrane permeability in electropermeabilization Title The contribution of lipid peroxidation to membrane permeability in electropermeabilization: A molecular dynamics study Author Rems, L. (TU Delft ChemE/Product and Process Engineering) Viano, Marilyne (Lorraine University) Kasimova, Marina A. (Lorraine University) Miklavčič, Damijan (University of Ljubljana) Tarek, Mounir (Lorraine University) Date 2019 Abstract Electroporation or electropermeabilization is a technique that enables transient increase in the cell membrane permeability by exposing cells to pulsed electric field. However, the molecular mechanisms of the long-lived cell membrane permeability, which persists on the minutes time scale after the pulse treatment, remain elusive. Experimental studies have suggested that lipid peroxidation could present a mechanism of this prolonged membrane permeabilization. In this study we make the first important step in quantifying the possible contribution of lipid peroxidation to electropermeabilization. We use free energy calculations to quantify the permeability and conductance of bilayers, containing an increasing percentage of hydroperoxide lipid derivatives, to sodium and chloride ions. We then compare our calculations to experimental measurements on electropermeabilized cells. Our results show that the permeability and conductance increase dramatically by several orders of magnitude in peroxidized bilayers. Yet this increase is not sufficient to reasonably account for the entire range of experimental measurements. Nevertheless, lipid peroxidation might be considered an important mechanism of prolonged membrane permeabilization, if exposure of cells to high voltage electric pulses leads to secondary lipid peroxidation products. Our analysis calls for experimental studies, which will determine the type and amount of lipid peroxidation products in electropermeabilized cell membranes. Subject Cell membraneElectrical conductanceElectroporationFree energy calculationsOxidized lipidsPermeability To reference this document use: http://resolver.tudelft.nl/uuid:d3226d61-7d9f-4c09-aa89-7dcd8824a021 DOI https://doi.org/10.1016/j.bioelechem.2018.07.018 Embargo date 2020-09-25 ISSN 1567-5394 Source Bioelectrochemistry, 125, 46-57 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2019 L. Rems, Marilyne Viano, Marina A. Kasimova, Damijan Miklavčič, Mounir Tarek Files PDF Manuscript_R2_tarek.pdf 4.68 MB Close viewer /islandora/object/uuid:d3226d61-7d9f-4c09-aa89-7dcd8824a021/datastream/OBJ/view