Print Email Facebook Twitter Alkaliphilic Life Title Alkaliphilic Life: Adaptation strategies by Caldalkalibacillus thermarum Author de Jong, S.I. (TU Delft BT/Environmental Biotechnology) Contributor van Loosdrecht, Mark C.M. (promotor) McMillan, D.G.G. (copromotor) Degree granting institution Delft University of Technology Date 2024-02-09 Abstract Alkaliphiles thrive in environments with a pH of 8.5 or above, while maintaining an internal pH closer to neutral. Thus, alkaliphilic microorganisms have a proton gradient inverted with respect to the normal orientation. Intuitively, this would nullify the potential to generate energy via respiration with regularly oriented respiratory chains that rely on proton-coupled ATP synthases. Yet, alkaliphilic respiratory chains are oriented traditionally and are actively used. The question therefore is how they are able to create conditions conducive to such behaviour. In addition, attempts to answer that question will hopefully also clarify how alkaliphiles acidify their cytoplasm with respect to the exterior milieu in the first place. This thesis details methods required to study these questions and provides some answers regarding alkaliphilic life. This thesis focuses on a single category of alkaliphiles: the low-salt gram positive alkaliphiles. These microbes have just a single membrane, the proteins therein, and a cell wall to generate conditions suitable for energy generation and other transport mechanisms. In short, it can be regarded as the most basic system to study an alkaline, or basic, problem.... Subject AlkaliphileMembraneGenomicsProteomicsLipidomics To reference this document use: https://doi.org/10.4233/uuid:ef90d088-f7ac-4767-a926-3b4bac9497e9 ISBN 978-94-6361-963-9 Part of collection Institutional Repository Document type doctoral thesis Rights © 2024 S.I. de Jong Files PDF SI_de_Jong_Dissertation_ISBN_V2.pdf 16.06 MB Close viewer /islandora/object/uuid:ef90d088-f7ac-4767-a926-3b4bac9497e9/datastream/OBJ/view