Print Email Facebook Twitter Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing Title Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing Author Rombouts, J.L. (TU Delft BT/Environmental Biotechnology; TU Delft OLD BT/Cell Systems Engineering) Mos, G. (TU Delft OLD BT/Cell Systems Engineering; TU Delft Applied Sciences) Weissbrodt, D.G. (TU Delft OLD BT/Cell Systems Engineering; TU Delft BT/Environmental Biotechnology) Kleerebezem, R. (TU Delft OLD BT/Cell Systems Engineering; TU Delft BT/Environmental Biotechnology) van Loosdrecht, Mark C.M. (TU Delft OLD BT/Cell Systems Engineering; TU Delft BT/Environmental Biotechnology) Faculty Applied Sciences Date 2019 Abstract A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or glucose in a sequencing batch reactor (SBR) or a continuous-flow stirred tank reactor (CSTR) mode. The combination of 16S rRNA gene-based analysis and fluorescence in situ hybridisation revealed no complete dominance of one species in the community. The glucose-fed and xylose-fed SBR enrichments were dominated >80% by one species. Enterobacteriaceae dominated the SBRs enrichments, with Citrobacter freundii dominant for xylose and Enterobacter cloacae for glucose. Clostridium, Enterobacteriaceae and Lachnospiraceae affiliates dominated the CSTRs enrichments. Independent of substrate, SBR communities displayed 2-3 times higher biomass-specific rate of substrate uptake (qsmax) and 50% lower biomass yield on ATP, to CSTR communities. Butyrate production was linked to dominance of Clostridium and low qsmax (1.06 Cmols Cmolx-1 h-1), while acetate and ethanol production was linked to dominance of Enterobacteriaceae and Lachnospiraceae and high qsmax (1.72 Cmols Cmolx-1 h-1 and higher). Overall, more diversity than expected through competition was observed, indicating mutualistic mechanisms might shape microbial diversity. To reference this document use: http://resolver.tudelft.nl/uuid:fcc46ebb-1367-4ad5-80d9-00e8132f2279 DOI https://doi.org/10.1093/femsec/fiy233 Embargo date 2019-12-03 ISSN 0168-6496 Source FEMS Microbiology Ecology, 95 (2) Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2019 J.L. Rombouts, G. Mos, D.G. Weissbrodt, R. Kleerebezem, Mark C.M. van Loosdrecht Files PDF 190204_Rombouts_et_al_201 ... _Delft.pdf 1.08 MB Close viewer /islandora/object/uuid:fcc46ebb-1367-4ad5-80d9-00e8132f2279/datastream/OBJ/view