Light-sheet optimization for microscopy

Conference paper (2016)

Authors

D. Wilding Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
P. Pozzi Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
O.A. Soloviev Flexible Optical B.V., Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Gleb Vdovin Flexible Optical B.V., Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
M.H.G. Verhaegen Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Research Group
Team Raf Van de Plas (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2016 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen
DOI: https://doi.org/10.1117/12.2210912
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Published Date

2016

Language

English

Reuse Rights

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Raf Van de Plas

Abstract

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.

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