In vivo analysis of Saccharomyces cerevisiae plasma membrane ATPase Pma1p isoforms with increased in vitro H+/ATP stoichiometry

Journal article (2012)
Department
BT/Biotechnology (AS) (TU Delft)
Copyright: This article is published with open access at Springerlink.com
DOI: https://doi.org/doi:10.1007/s10482-012-9730-2
More Info
expand_more

Published Date

10-04-2012

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Source:

Antonie van Leeuwenhoek, 102 (2), 2012

Faculty

Applied Sciences

Department

BT/Biotechnology

Abstract

Plasma membrane H+-ATPase isoforms with increased H+/ATP ratios represent a desirable asset in yeast metabolic engineering. In vivo proton coupling of two previously reported Pma1p isoforms (Ser800Ala, Glu803Gln) with increased in vitro H+/ATP stoichiometries was analysed by measuring biomass yields of anaerobic maltose-limited chemostat cultures expressing only the different PMA1 alleles. In vivo H+/ATP stoichiometries of wildtype Pma1p and the two isoforms did not differ significantly.