Robust experimental methods to study in-vivo pre-steady state kinetics of primary metabolism in Saccharomyces cerevisiae

Doctoral thesis (2005)

Authors

Contributors

J.J. Heijnen (promotor)
Faculty
Applied Sciences (AS) (TU Delft)
Copyright: © 2005 M.R. Mashego
Saccharomyces cerevisiae Chemostat culture Metabolomics Glucose perturbations Bioscope
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Published Date

03-11-2005

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ISBN:

90-902-0060-6

Faculty

Applied Sciences

Abstract

In this thesis, robust experimental methods and experimental designs for quantitative analysis of the metabolome (concentrations of relevant metabolites in the cell) of Saccharomyces cerevisiae (Bakers yeast) have been presented. In this research, it is essential to obtain a snapshot of intracellular as well as extracellular metabolite concentrations under defined conditions and as a function of time. Therefore, it is necessary to obtain within a fraction of a second, a representative sample from the population of cells and instantly arrest cellular metabolism.