Immobilization of the peroxygenase from agrocybe aegerita. The effect of the immobilization ph on the features of an ionically exchanged dimeric peroxygenase

Carballares, Diego; Morellon-Sterling, Roberto; Xu, X.; Hollmann, F.; Fernandez-Lafuente, Roberto

doi:10.3390/catal11050560

Immobilization of the peroxygenase from agrocybe aegerita. The effect of the immobilization ph on the features of an ionically exchanged dimeric peroxygenase

Title

Immobilization of the peroxygenase from agrocybe aegerita. The effect of the immobilization ph on the features of an ionically exchanged dimeric peroxygenase

Author

Carballares, Diego (Campus de Cantoblanco)
Morellon-Sterling, Roberto (Campus de Cantoblanco)
Xu, X. (TU Delft BT/Biocatalysis)
Hollmann, F. (TU Delft BT/Biocatalysis)
Fernandez-Lafuente, Roberto (King Abdulaziz University; Campus de Cantoblanco)

Date

2021

Abstract

This paper outlines the immobilization of the recombinant dimeric unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). The enzyme was quite stable (remaining unaltered its activity after 35 h at 47^◦C and pH 7.0). Phosphate destabilized the enzyme, while glycerol stabilized it. The enzyme was not immobilized on glyoxyl-agarose supports, while it was immobilized albeit in inactive form on vinyl-sulfone-activated supports. rAaeUPO immobilization on glutaraldehyde pre-activated supports gave almost quantitative immobilization yield and retained some activity, but the biocatalyst was very unstable. Its immobilization via anion exchange on PEI supports also produced good immobilization yields, but the rAaeUPO stability dropped. However, using aminated agarose, the enzyme retained stability and activity. The stability of the immobilized enzyme strongly depended on the immobilization pH, being much less stable when rAaeUPO was adsorbed at pH 9.0 than when it was immobilized at pH 7.0 or pH 5.0 (residual activity was almost 0 for the former and 80% for the other preparations), presenting stability very similar to that of the free enzyme. This is a very clear example of how the immobilization pH greatly affects the final biocatalyst performance.

Subject

Effect of immobilization medium on enzyme immobilized stability
Enzyme immobilization
Enzyme stability
Ionic exchange

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http://resolver.tudelft.nl/uuid:87efbc18-9c2b-45c9-bca4-baae372f326d

DOI

https://doi.org/10.3390/catal11050560

ISSN

2073-4344

Source

Catalysts, 11 (5)

Part of collection

Institutional Repository