Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels

Journal article (2016)

Authors

Victor Marin Kavli institute of nanoscience Delft
R.Y. Kieffer Kavli institute of nanoscience Delft, BN/Marie-Eve Aubin-Tam Lab - AS
Raymond Padmos Kavli institute of nanoscience Delft
M.E. Aubin-Tam Kavli institute of nanoscience Delft, BN/Marie-Eve Aubin-Tam Lab - AS
Research Group
BN/Marie-Eve Aubin-Tam Lab (AS) (TU Delft)
Copyright: © 2016 Victor Marin, R.Y. Kieffer, Raymond Padmos, M.E. Aubin-Tam
DOI: https://doi.org/10.1021/acs.analchem.6b00926
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Published Date

28-06-2016

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Marie-Eve Aubin-Tam Lab

Abstract

We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.