Design of a miniature gas exchanger for oxygen control in microfluids

Design, integration, fabrication and validation of a miniature cell medium gas exchanger to control (sub)physiological oxygen concentrations on a liver chip

Master thesis (2021)

Authors

M.P. van Reeuwijk Mechanical Engineering

Contributors

U. Staufer Micro and Nano Engineering - Mechanical, Maritime and Materials Engineering (mentor)
Matthijs Langelaar Computational Design and Mechanics - Mechanical, Maritime and Materials Engineering (mentor)
N.J.H. Raat Dynamics of Micro and Nano Systems - Mechanical, Maritime and Materials Engineering (mentor)
Faculty
Mechanical Engineering, Mechanical Engineering
Copyright: © 2021 Matthijs van Reeuwijk
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Published Date

22-07-2021

Language

English

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Faculty

Mechanical Engineering

Abstract

Current technology of Organ-on-Chips allows investigation of human cells outside the body including a microfluidic medium flow. Control of oxygen in this microfluidic flow is required to mimic the situation as in the human liver (physiological) or to create temporary low oxygen concentrations (hypoxia). In this research, a gas exchanger module is designed for liver-on-a-chip cell experiments at the Erasmus MC. Based on prototype tests and COMSOL simulations, the gas exchanger module is designed and integrated with a commercial Micronit microfluidic channel. The functioning of the fabricated gas exchanger is validated by measuring an oxygen gradient as well as performing oxygen consumption measurements on a liver cell layer.