Biogenic reefs form biodiversity hotspots and are key components of marine ecosystems, making them priority habitats for nature conservation. However, the conservation status of biogenic reefs generally depends on their size and stability. Dynamic, patchy reefs may therefore be excluded from protection. Here, we studied epibenthos and epifauna density, richness, and community composition of patchy, dynamic Sabellaria spinulosa (ross worm) reefs in the North Sea. This study was conducted by comparing boxcore (endobenthos) and video transect (epifauna) data from two research campaigns in 2017 and 2019 to the Brown Bank area on the Dutch Continental Shelf, where S. spinulosa reefs were first discovered in 2017. The Brown Bank area is characterized by dynamic, migratory bedforms at multiple scales which potentially affect biogenic reef stability. We showed that S. spinulosa habitats had a patchy distribution and alternated with habitats comprised of plain sand. Average S. spinulosa habitat patch size was 5.57 ± 0.99 m and 3.94 ± 0.22 m in 2017 and 2019, respectively (mean ± SE), which especially in 2019 closely resembled the small-scale megaripple bedforms. Contrary to the endobenthos communities that were unaffected by S. spinulosa, epifauna density and species richness were at least two times higher in S. spinulosa habitats compared to sandy habitats, resulting in different community compositions between the two habitat types. We showed that S. spinulosa persisted in the area for almost 2 years. Although the stability of individual patches remained unclear, we demonstrated that even patchy biogenic reefs may promote density and local biodiversity of mobile, epibenthic species, very likely as a result of increased habitat heterogeneity provided by reef habitat patches. This indicates that patchy biogenic reefs that occur in dynamic environments may also have high ecological value and their conservation status should be (re)considered to ensure their protection.

Conflicts of interests between economic and nature conservation stakeholders are increasingly common in coastal seas, inducing a growing need for evidence-based marine spatial planning. This requires accurate, high-resolution habitat maps showing the spatial distribution of benthic assemblages and enabling intersections of habitats and anthropogenic activities. However, such detailed maps are often not available because relevant biological data are scarce or poorly integrated. Instead, physiotope maps, solely based on abiotic variables, are now often used in marine spatial planning. Here, we investigated how pointwise, relatively sparse biological data can be integrated with gridded, high-resolution environmental data into informative habitat maps, using the intensively used southern North Sea as a case-study. We first conducted hierarchical clustering to identify discrete biological assemblages for three faunal groups: demersal fish, epifauna, and endobenthos. Using Random Forest models with high-resolution abiotic predictors, we then interpolated the distribution of these assemblages to high resolution grids. Finally, we quantified different anthropogenic pressures for each habitat. Habitat maps comprised a different number of habitats between faunal groups (6, 13, and 10 for demersal fish, epifauna, and endobenthos respectively) but showed similar spatial patterns for each group. Several of these ‘fauna-inclusive’ habitats resembled physiotopes, but substantial differences were also observed, especially when few (6; demersal fish) or most (13; epifauna) physiotopes were delineated. Demersal fishing and offshore wind farms (OWFs) were clearly associated with specific habitats, resulting in unequal anthropogenic pressure between different habitats. Natura-2000 areas were not specifically associated with demersal fishing, but OWFs were situated mostly inside these protected areas. We thus conclude that habitat maps derived from biological datasets that cover relevant faunal groups should be included more in ecology-inclusive marine spatial planning, instead of only using physiotope maps based on abiotic variables. This allows better balancing of nature conservation and socio-economic interests in continental shelf seas.

High-resolution surveying techniques of subtidal soft-bottom seafloor habitats show higher small-scale variation in topography and sediment type than previously thought, but the ecological relevance of this variation remains unclear. In addition, high-resolution surveys of benthic fauna show a large spatial variability in community composition, but this has yet poorly been linked to seafloor morphology and sediment composition. For instance, on soft-bottom coastal shelves, hydrodynamic forces from winds and tidal currents can cause nested multiscale morphological features ranging from metre-scale (mega)ripples, to sand waves and kilometre-scale linear sandbanks. This multiscale habitat heterogeneity is generally disregarded in the ecological assessments of benthic habitats. We therefore developed and tested a novel multiscale assessment toolbox that combines standard bathymetry, multibeam backscatter classification, video surveying of epibenthos and box core samples of sediment and macrobenthos. In a study on the Brown Bank, a sandbank in the southern North Sea, we found that these methods are greatly complementary and allow for more detail in the interpretation of benthic surveys. Acoustic and video data characterised the seafloor surface and subsurface, and macrobenthos communities were found to be structured by both sandbank and sand wave topography. We found indications that acoustic techniques can be used to determine the location of epibenthic reefs. The multiscale assessment toolbox furthermore allows formulating recommendations for conservation management related to the impact of sea floor disturbances through dredging and trawling.

Seafloor characterization using multibeam echosounder (MBES) backscatter data is an active field of research. The observed backscatter curve (OBC) is used in an inversion algorithm with available physics-based models to determine the seafloor geoacoustic parameters. A complication is that the OBC cannot directly be coupled to the modeled backscatter curve (MBC) due to the correction of uncalibrated sonars. Grab samples at reference areas are usually required to estimate the angular calibration curve (ACC) prior to the inversion. We first attempt to estimate the MBES ACC without grab sampling by using the least squares cubic spline approximation method implemented in a differential evolution optimization algorithm. The geoacoustic parameters are then inverted over the entire area using the OBCs corrected for the estimated ACC. The results indicate that a search for at least three geoacoustic parameters is required, which includes the sediment mean grain size, roughness parameter, and volume scattering parameter. The inverted mean grain sizes are in agreement with grab samples, indicating reliability and stability of the proposed method. Furthermore, the interaction between the geoacoustic parameters and Bayesian acoustic classes is investigated. It is observed that higher backscatter values, and thereby higher acoustic classes, should not only be attributed to (slightly) coarser sediment, especially in a homogeneous sedimentary environment such as the Brown Bank, North Sea. Higher acoustic classes should also be attributed to larger seafloor roughness and volume scattering parameters, which are not likely intrinsic to only sediment characteristics but also to other contributing factors.

High resolution maps of sandy seafloors are valuable to understand seafloor dynamics, plan engineering projects, and create detailed benthic habitat maps. This paper presents multibeam echosounder backscatter classification results of the Brown Bank area of the North Sea. We apply the Bayesian classification method in a megaripple and sand wave area with significant slopes. Prior to the classification, corrections are implemented to account for the slopes. This includes corrections on the backscatter value and its corresponding incident angle. A trade-off in classification resolutions is found. A higher geo-acoustic resolution is obtained at the price of losing spatial resolution, however, the Bayesian classification method remains robust with respect to these trade-off decisions. The classification results are compared to grab sample particle size analysis and classified video footage. In non-distinctive sedimentary environments, the acoustic classes are not attributed to only the mean grain size of the grab samples but to the full spectrum of the grain sizes. Finally, we show the Bayesian classification results can be used to characterize the sedimentary composition of megaripples. Coarser sediments were found in the troughs and on the crests, finer sediments on the stoss slopes and a mixture of sediments on the lee slopes.

The tube-building polychaete Sabellaria spinulosa (Ross worm) can form conspicuous biogenic reefs that stabilize the seabed and increase biodiversity by providing a habitat for a multitude of other species. These reefs, however, are assumed to be vulnerable to human-induced physical disturbances of the seabed. In the Greater North Sea, S. spinulosa reefs are recognized to be under threat and worthy of protection. In August 2017, three S. spinulosa reefs with a minimum extent of 1016 m² were discovered in the Dutch Brown Bank area. This area comprises a large-scale sandbank and adjacent troughs. The reefs were found within the sandbank troughs, which have proven to be subject to high demersal fishing intensities (fished >5 times a year). Detailed bathymetry measurements showed that S. spinulosa reefs were mainly located within valleys of smaller-scaled sand waves, which have a perpendicular orientation compared to the large-scale sandbank structure of the Brown Bank. We hypothesize that the valleys in between sand waves offer suitable substrate for settlement and refuge from abrasion by fishing activities, enabling the S. spinulosa reefs to persist despite high fishing intensities. ROV footage of the reefs showed higher estimates of species abundances on the reefs compared with adjacent habitats, with some species present that are typical for hard substrate (rock gunnel, Pholis gunnellus; edible crab, Cancer pagurus; and velvet swimming crab, Necora puber). The information presented could be used for drafting management policies to protect these reefs, as Contracting Parties of the OSPAR Convention are committed to take measures and protect biodiversity.

The sandy seabed of shallow coastal shelf seas displays morphological patterns of various dimensions. The seabed also harbors a rich ecosystem. Increasing pressure from human-induced disturbances necessitates further study on drivers of benthic community distributions over morphological patterns. Moreover, a greater understanding of the sand ripple distribution over tidal sand waves may improve morphological model predictions. Here we analyzed the biotic abundance and ripple morphology in sand wave troughs and crests using video transects. We found that both the epibenthos and endobenthos are significantly more abundant in sand wave troughs, where ripples are less abundant and more irregularly shaped. Finally, we show that camera systems are relatively quick and effective tools to study biotic spatial patterns in relation to seabed morphology.