Before the operation of newly constructed railways, tamping and stabilizing machines should be used to improve the quality of ballast beds. With the expansion of the railway network and increase of speeds and axle loads, higher quality and efficiency for tamping and stabilizing operation are required. However, previous studies did not involve the effects and parameters of three-sleeper tamping and stabilizing operation under complex working conditions. In the paper, the effect of a three-sleeper tamping and stabilizing machine on the ballast bed state has been studied by performing field experiments. The effect of important factors, including tamping modes, stabilizing frequency, and track lifting amount, are discussed in detail. The results show that the tamping operation on newly constructed railways causes a reduction of the lateral resistance by 56.5 % and a reduction of lateral resistance work by 64.9 %. After the stabilizing operation, the lateral resistance and lateral resistance work are increased by 168.6 % and 209.8 %, respectively. The tamping and stabilizing operation can significantly increase the support stiffness of ballast beds, which meets the requirements of train operation. Meanwhile, 2X tamping mode is more beneficial to improve ballast resistance. Besides, it is reasonable for a stabilizing frequency of 25 Hz to be used for newly constructed railways. The track lifting amount also has a large effect on the ballast bed quality, and it is recommended to keep the lift amount in the range of 20 mm ∼ 30 mm to achieve a better tamping quality.