Mars Express (MEX) is one of the most productive planetary missions of the European Space Agency (ESA). This low cost (∼150 M€) mission has been instrumental in shaping the planetary community in Europe and has contributed to paving the way for many subsequent ESA endeavours. During more than two decades, Mars Express has collected a wealth of data in all disciplines of Martian science. This paper concludes the Topical Collection “Mars Express: Pioneering Two Decades of European Science and Exploration of Mars” prepared under the auspices of the International Space Science Institute. It briefly describes various aspects of the mission (leaving details to dedicated articles), summarizes the major science achievements, discusses the lessons learned from 20 years of Mars Express operations, and bridges with future Mars science and exploration.

Spacecraft-to-spacecraft radio occultations experiments are being conducted at Mars between Mars Express (MEX) and Trace Gas Orbiter (TGO), the first ever extensive inter-spacecraft occultations at a planet other than Earth. Here we present results from the first 83 such occultations, conducted between 2 Nov 2020 and 5th of July 2023. Of these, 44 observations have to-date resulted in the extraction of vertical electron density profiles. These observations are the successful results of a major feasibility study conducted by the European Space Agency to use pre-existing relay communication equipment for radio science purposes. Mutual radio occultations have numerous advantages over traditional spacecraft-to-ground station occultations. In this work, we demonstrate how raw data are transformed into electron density values and validated with models and other instruments.