The unambiguous determination of the chemical functionality over graphene oxide (GO) is important to unleash its potential applications. However, the mapping of oxygen functionalities distribution remains to be unequivocally determined because of highly inhomogeneous non-stoichiometric structures and ultra-thin layers of GO. In this study, we report an experimental observation of the spatial distribution of oxygen functional groups on monolayer and multilayer GO using AFM-IR, atomic force microscopy coupled with infrared spectroscopy. Overcoming conventional IR diffraction limit for several micrometers, the novel AFM-IR reaches high spatial resolution ∼20 nm and could detect IR absorption on ∼1 nm thickness of monolayer GO. With nanoscale chemical mapping, the distribution of different oxygen functional groups is distinguished with AFM-IR over the GO surface. It allows us to observe that these oxygen functional groups prefer to sit on the fold areas, in discrete domains and on the edges of GO, which gave more insights into its chemical nature. The determination of the position of functional groups through precise imaging contributes to our understanding of GO structure-properties relations and paves the way for targeted tethering of polymers, biomaterials, and other nanostructures.@en

Interactions between graphene oxide (GO) and organic molecules play a role in processes such as environmental remediation and water treatment. However, little is known about underlying molecular level processes with the presence of ions. In this study, we utilized atomic force microscopy (AFM) in chemical force mapping (CFM) mode to directly probe their adhesion interactions. AFM tips were functionalised to serve as models for nonpolar and polar organic molecules, i.e. with alkyl, -CH3, and carboxyl, -COO(H). For experiments with -COO(H) tips, adhesion between GO and tips decreased in the order: Ba2+ > Ca2+ > Mg2+ > Na+, whereas for the -CH3 tips, ion dependent adhesion was relatively low but followed the same: Ba2+ > Ca2+ > Mg2+ ≈ Na+. Calculations with Derjaguin-Landau-Verwey-Overbeek (DLVO) theory and the Schulze-Hardy rule could not account for the observations. We propose that ion bridging plays a definitive role in adhesion between -COO(H) tips and the GO surface. This is consistent with proposed models with density functional theory (DFT) calculations. Adhesion of -CH3 tips is a response to the hydrophilic interactions and the ion dependent part is suggested to arise from ion bridging between slightly negative charged -CH3 tips and the GO surface. High pH had a notable influence on the adhesion of the -COO(H) tip but a negligible effect on the -CH3 tip. These results offer important insights into interactions between solutions and mineral surfaces with adsorbed organic molecules.@en