In-vehicle technologies and co-operative services have potential to ease congestion problems and improve traffic safety. This paper investigates the impact of infrastructure-to-vehicle co-operative systems, case of CO-OPerative SystEms for Intelligent Road Safety (COOPERS), on driver behavior. Thirty-five test drivers drove an instrumented vehicle, twice, with and without the system. Data related to driving behavior, physiological measurements, and user acceptance was collected. A macro-level approach was used to evaluate the potential impact of such systems on driver behavior and traffic safety. The results in terms of speeds, following gaps, and physiological measurements indicate a positive impact. Furthermore, drivers' opinions show that the system is in general acceptable and useful.